Project description:We undertook to determine whether carcinoma cells residing in the epithelial or quasi-mesenchymal phenotypic states differ in their expression of immunomodulatory markers. To do so, we used the nCounter PanCancer Immune Profiling Panel (Nanostring Technologies)to perform transcriptomic analyses of the E and qM mammary carcinoma cells; these cells were either cultured in vitro or prepared by FACS sorting of GFP-labelled carcinoma cells from their corresponding tumors
Project description:The gene expression analysis of the RNA samples were analyzed with NanoString Non Human Primate immunology panel comprised of 754 immune related gene
Project description:Murine 4T1 breast cancer was grown in vivo and treated with repeat intratumoral influenza vaccine. Flash frozen tissue evaluated by nCounter mouse immune exhaustion panel.