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Epigenome analysis of glycine chloramine treated Jurkat T cells

ABSTRACT: Epigenome analysis of glycine chloramine treated Jurkat T cells

PROVIDER: PRJNA815983 | ENA |

REPOSITORIES: ENA

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Epigenome analysis of glycine chloramine treated Jurkat T cells

Project description:Genome wide DNA methylation profiling of Jurkat T lymphoma cell lines, using the human DNA methylationEPIC 850K array. All cells were blocked using a thymidine block, and DNA methylation was analysed 4 hrs post release from the block (S-Phase) or 72 hours later. Samples are either untreated control (PBS) or treated with Glycine chloramine. Cells were exposed to Glycine chloramine in two independent time points: pre replication (same time as release from block, 3 replicates) or during S-phase (four replicates, 2 hrs post block release). These two exposures were assessed in two independent experiments and therefore have their own controls. Each replicate used cells from a different passage number, and was performed independently.

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Project description:Epigenome and gene expression analysis of glycine chloramine treated Jurkat T cells

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Glycine Chloramine directed gene expression changes in Jurkat T cell lines

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Epigenome and gene expression analysis of glycine chloramine treated Jurkat T cells

Project description:This SuperSeries is composed of the SubSeries listed below.

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Differential expression of miRNA in control and CD3/CD28mAb-treated Jurkat cells

Project description:The cultured cell line Jurkat is frequently employed in studies of T cell function. Here we identified the microRNAs expressed in Jurkat cells in the presence and absence of CD3/CD28mAb treatment. Analyzed the expression of microRNAs extracted from untreated Jurkat cells as a control and Jurkat cells treated with CD3/CD28mAb.

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