Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [LCM Tuba-seq]

ABSTRACT: A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [LCM Tuba-seq]

PROVIDER: PRJNA816203 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer

Project description:RNA-seq, LCM sequencing, and Tuba-seq data for the article titled, "A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer." This SuperSeries is composed of the SubSeries listed below.

2023-01-09 | GSE198637 | GEO

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [SCLC Tuba-seq]

Project description:A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [SCLC Tuba-seq]

| PRJNA816202 | ENA

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [LCM Tuba-seq]

Project description:We generated a model of lentivirus-initiated mouse small cell lung cancer (SCLC) combined with CRISPR/Cas9 knockout and barcoding. We analyzed tumor suppressive functions of 39 gene candidates using these in vivo CRISPR screening approaches and found Tsc1 to be one of the top tumor suppressor genes in SCLC.

2023-01-09 | GSE198635 | GEO

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer

Project description:A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer

| PRJNA816196 | ENA

Tuba metagenome

Project description:Tuba metagenome Raw sequence reads

| PRJNA527288 | ENA

A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [RNAseq]

Project description:A multiplexed in vivo gene knockout approach to identify cancer drivers in small cell lung cancer [RNAseq]

| PRJNA816199 | ENA

Macrostomum tuba

Project description:Macrostomum tuba Transcriptome or Gene expression

| PRJNA379126 | ENA

Multiplexed Single Cell gene expression profile of barcoded SUM159PT cells

Project description:Cancer is a heterogeneous disease, where multiple, phenotypically distinct subpopulations co-exist. Tumour evolution is a result of a complex interplay of genetic and epigenetic factors. To predict the molecular drivers of distinct cancer responses, we apply single-cell lineage tracing (scRNA-Seq of barcoded cells) on a triple-negative breast cancer model. SUM159PT cells infected with a lentiviral barcode library (Perturb-seq Library) were sorted according to the presence of BFP signal, treated or not with paclitaxel (PTX), multiplexed with MULTI-Seq protocol, and then processed by scRNA-Seq.

2024-05-24 | E-MTAB-13066 | biostudies-arrayexpress

In vivo multiplexed screen reveals a critical role of Keap1/Nrf2 pathway in small cell lung cancer [TUBAseq]

Project description:Approximately 15% of all lung cancer cases are small cell lung cancer (SCLC), which originates from neuroendocrine cells lining the airways (bronchi) of the lung. SCLC is known for its aggressive nature and swift growth, resulting in a five-year survival rate of around 6%. Understanding the genetic pathways that drive tumor development remains an ongoing challenge. In our study, we employed an in vivo multiplexed approach, known as Tuba-seq, to investigate the impact of the functional loss of 18 putative tumor suppressors. Our screening unveiled Pten and Keap1 as top candidates; knocking out either one promoted both tumor initiation and progression. We identified the Keap1/Cul3/Nrf2 pathway as a pivotal regulator for SCLC development, particularly in regulating the susceptibility of SCLC to ferroptosis. Our work not only established an in vivo multiplexed approach for assessing the role of tumor suppressors in SCLC but also uncovers a previously unappreciated role of Keap1 in SCLC.

2024-01-26 | GSE239440 | GEO

In vivo multiplexed screen reveals a critical role of Keap1/Nrf2 pathway in small cell lung cancer [RNAseq]

2024-01-26 | GSE239436 | GEO