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Lichen secondary metabolites inhibit Wnt/β-catenin pathway in glioblastoma cells and improve the anticancer effects of temozolomide

ABSTRACT: Lichen secondary metabolites inhibit Wnt/β-catenin pathway in glioblastoma cells and improve the anticancer effects of temozolomide

PROVIDER: PRJNA818711 | ENA |

REPOSITORIES: ENA

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Lichen secondary metabolites inhibit Wnt/β-catenin pathway in glioblastoma cells and improve the anticancer effects of temozolomide

Project description:Lichens are a source of secondary metabolites with significant pharmacological potential. Data regarding their possible application in glioblastoma (GBM) treatment are, however scarce. The study aimed to analyze the mechanism of action of six lichen secondary metabolites: atranorin, caperatic acid, physodic acid, squamatic acid, salazinic acid, and lecanoric acid using two- and three-dimensional GBM cell line models. The Parallel Artificial Membrane Permeation Assay was used to predict the blood-brain barrier penetration ability of the tested compounds. Their cytotoxicity was analyzed using MTT test on A-172, T98G, and U-138 MG cells. Flow cytometry was applied for the analysis of oxidative stress, cell cycle distribution, and apoptosis, whereas qPCR and microarrays detected the induced transcriptomic changes. Our data confirm the ability of lichen secondary metabolites to cross the blood-brain barrier and exert cytotoxicity against GBM cells. Moreover, the compounds generated oxidative stress, interfered with the cell cycle, and induced apoptosis in T98G cells. They also inhibited Wnt/β-catenin pathway, and this effect was even stronger in case of a co-treatment with temozolomide. Transcriptomic changes in cancer related genes induced by caperatic acid and temozolomide were the most pronounced. Lichen secondary metabolites, in particular caperatic acid should be further analyzed as potential anti-GBM agents.

2022-03-23 | GSE199185 | GEO

Transcrpitomic analysis of Oral lichen planus with immumosuppressive therapy

Project description:We compared the transcriptomes of tissues from Oral lichen planus patients with immunosuppressive therapy to reveal the biological mechanism of oral lichen planus treatment.

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Gene profile of glioblastoma cells treated with y15 and temozolomide

Project description:The gene expression profiles were identified in glioblastoma cells treated with FAK inhibitor Y15, temozolomide alone or with combination of Y15 and Temozolomide DBTRG and U87 were treated with FAK inhibitor Y15 at 10 microM for 24 h; U87 cells were treated with Temozolomide 100 microM for 24 h and Y15+temozolomide at the same dose as each agent alone

2013-01-12 | E-GEOD-43452 | biostudies-arrayexpress

Gene expression profiling of oral and genital lichen planus epithelium and normal oral and genital epithelium

Project description:Comparing gene expression in Oral and genital lichen planus with normal oral and genital epithelium trying to idenitfy differently expressed genes in lichen planus compared to normal epithelium Total RNA obtained from oral and genital lichen planus epithelium compared with normal oral and genital epithelium

2013-11-08 | E-GEOD-52130 | biostudies-arrayexpress

Novel Dopamine Receptor 3 Antagonists Inhibit the Growth of Primary and Temozolomide Resistant Glioblastoma Cells

Project description:Novel Dopamine Receptor 3 Antagonists Inhibit the Growth of Primary and Temozolomide Resistant Glioblastoma Cells

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Profiling of Salivary Exosomal microRNAs in Oral Lichen Planus Patients [set 2]

Project description:In this study, we compared microRNA (miRNA) profiles of salivary exosomes of patients with oral lichen planus with those of healthy controls. Saliva samples from 16 patients with oral lichen planus and 8 healthy controls were divided into 2 sets and were examined by performing miRNA microarray analysis. Examination of 8 oral lichen planus patients and 4 healthy controls. Each patient and control represent pooled RNAs from salivary exosomes of 8 patients and 4 healthy controls, respectively. Please note that each set (i.e. set1 and set2) was analysed independently.

2015-04-30 | E-GEOD-68407 | biostudies-arrayexpress

Profiling of Salivary Exosomal microRNAs in Oral Lichen Planus Patients [set 1]

2015-04-30 | E-GEOD-68406 | biostudies-arrayexpress

Profiling of Salivary Exosomal microRNAs in Oral Lichen Planus Patients [set 2]

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Profiling of Salivary Exosomal microRNAs in Oral Lichen Planus Patients [set 1]

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lichen metagenome

Project description:Lichen Metagenome

| PRJNA834798 | ENA