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Transcriptome profiling of Arabidopsis roots upon lateral root induction in WT and TOR knock down [dataset1]

ABSTRACT: Transcriptome profiling of Arabidopsis roots upon lateral root induction in WT and TOR knock down [dataset1]

PROVIDER: PRJNA818879 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18441276.fastq.gz	Fastqsanger.gz
	SRR18441277.fastq.gz	Fastqsanger.gz
	SRR18441278.fastq.gz	Fastqsanger.gz
	SRR18441279.fastq.gz	Fastqsanger.gz
	SRR18441280.fastq.gz	Fastqsanger.gz

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Transcriptome profiling of Arabidopsis roots upon lateral root induction in WT and TOR knock down [dataset1]

Project description:To get a genome wide picture of the effects of TOR knockdown during the early phase of lateral root formation, we compared by RNA-seq the transcriptomes of roots 6h after the synchronous induction of lateral formation by auxin treatment (Himanen et al, 2002 - http://dx.doi.org/10.1105/tpc.004960) in the inducible UB10pro>>amiR-TOR background.

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Profiling of bulk and ribosome associated mRNA of Arabidopsis roots upon lateral root induction upon TOR activity inhibition with AZD8055 [dataset2]

Project description:We performed targeted purification of polysomal mRNA (TRAP-Seq, Vragović et al, 2015 - http://dx.doi.org/10.1073/pnas.1417947112) using a transgenic line ubiquitously expressing a GFP-tagged RPL18 (Mustroph et al, 2009 - https://doi.org/10.1073/pnas.0906131106) 6h after the synchronous induction of lateral root formation by auxin treatment (IAA) upon inhibition of TOR activity (AZD8055 treatment, AZD). To correct for abundance of mRNA, bulk RNA-Seq was performed on the same samples and used to normalise the reads purified with the ribosomes.

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Project description:To identify genes involved in the early phases of lateral root initiation, we profiled the transcriptomes of plants synchronously induced for lateral root initiation after 0, 1, 2, 4 and 6h of auxin treatment in conditions where IAA14 or IAA3-dependent auxin signaling is blocked. For this we used seedlings expressing non-degradable versions of the AUX/IAAs IAA14 (slr-1) or IAA3 (shy2-2) fused to the glucocorticoid receptor domain (slr-1:GR or shy2-2:GR) under the control of the pericycle and founder cell specific GATA23 promoter. Treatment with dexamethasone induces, specifically in pericycle cells, the nuclear translocation of the non-degradable AUX/IAA that acts as a dominant repressor of auxin signaling resulting in a complete block of lateral root formation

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