Project description:In this study, we constructed a tissue-engineered DRG-CSC (corneal stromal cells) co-cultured model (DS model), a CSC monoculture model (S model), and a DRG monoculture model (D model). Chicken DRGs were extracted from E7-E10 chicken embryo. Human CSCs were extracted in stromal lenticules from small incision lenticule extraction (SMILE). Transcriptional profiling of DRG in the DS model (DS-D), DRG in the D model(D-D), CSCs in the DS model (DS-S), CSCs in the S model (S-S) was analyzed. After 7 days of culture, RNA of each group was extracted.
Project description:Comparative RNA-Seq profiling of Sideroxydans sp. CL21, a microaerophilic, Fe(II)-oxidizer, and the facultative anaerobe Shewanella oneidensis, an Fe(III) reducer. The microorganisms were grown in co-culture and monoculture batch incubations under microaerobic growth conditions. RNA-Seq profiling was used to compare the transcriptomes of both Sideroxydans sp. CL21 and S. oneidensis when grown in co-culture compared to growth in monoculture
Project description:Proteomic analysis revealed that expression of 120, 98, and 138 kinds of protein were increased by more than two-fold in culture medium of hiPS-gut monoculture, PXB-cells monoculture, and co-culture condition compared with non-treatment medium, respectively.
Project description:To identify the concurrent effects of multiple environmental factors on gene expression under natural climatic fluctuations in rice, we examined global gene expression patterns in two fields typical of the main modes of rice cultivation, over two seasons, each comprising 15 time-points in three different genotypes Two time-series of 15 time-points, one for each season (dry and wet) over two different fields
Project description:To identify the concurrent effects of multiple environmental factors on gene expression under natural climatic fluctuations in rice, we examined global gene expression patterns in two fields typical of the main modes of rice cultivation, over two seasons, each comprising 15 time-points in three different genotypes
Project description:This set of microarray data was used to compare the effects of D. vulgaris grown syntrophically with a hydrogenotrophic methanogen versus D. vulgaris grown in sulfate-limited monoculture. Keywords: physiological response, one time point (stable continuous culture for both control and treated cells)
Project description:We use a human whole genome microarray to analyze the effects of nanosecond pulsed electric fields on Jurkat cells with the focus on early response genes to DNA damage. Keywords: nanosecond pulsed electric fields, jurkat cells, DNA damage