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Single cell RNA sequencing of Gipr- and Glp1r-expressing cells in the mouse hypothalamus

ABSTRACT: Single cell RNA sequencing of Gipr- and Glp1r-expressing cells in the mouse hypothalamus

PROVIDER: PRJNA819265 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18395823.fastq.gz	Fastqsanger.gz
	SRR18395824.fastq.gz	Fastqsanger.gz
	SRR18395825.fastq.gz	Fastqsanger.gz
	SRR18395826.fastq.gz	Fastqsanger.gz
	SRR18395827.fastq.gz	Fastqsanger.gz

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Transcriptomics of Gipr-expressing cells in mouse hypothalamus tissue [dataset 2]

Project description:We purified Gipr-expressing cells from mouse hypothalamus by FACS and identified cell types distinguished by transcripts.

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Transcriptomics of Gipr-expressing cells in mouse hypothalamus tissue [dataset 1]

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Glucose-dependent Insulinotropic polypeptide receptor-expressing cells in the hypothalamus regulate food intake

Project description:Ambiguity regarding the role of glucose-dependent insulinotropic polypeptide (GIP) in obesity arises from conflicting reports asserting that both GIP receptor (GIPR) agonism and antagonism are effective strategies for inhibiting weight gain. To enable identification and manipulation of Gipr-expressing (Gipr) cells we created GIPR-Cre knock-in mice. As GIPR-agonists have recently been reported to suppress food intake we aimed to identify central mediators of this effect. Gipr cells were identified in the arcuate, dorsomedial, and paraventricular nuclei of the hypothalamus, as confirmed by RNAscope in mouse and human. Single cell RNAseq identified clusters of hypothalamic Gipr cells exhibiting transcriptomic signatures for mural, glial and neuronal cells, the latter expressing somatostatin, but little proopiomelanocortin or agouti-related peptide. Activation of Gq-DREADDs in hypothalamic Gipr cells suppressed food intake in vivo, which was not obviously additive with concomitant GLP1R activation. These data identify hypothalamic GIPR as a target for the regulation of energy balance.

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Project description:We purified Glp1r-expressing cells from mouse hypothalamus by FACS and identified cell types distinguished by transcripts.

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Transcriptomics of Glp1r-expressing cells in mouse hypothalamus tissue

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Hypothalamic and dorsal vagal glucose-dependent insulinotropic polypeptide receptor (GIPR) neurons employ distinct mechanisms to affect feeding

Project description:Central glucose-dependent insulinotropic polypeptide (GIP) receptor (GIPR) signalling is critical for GIP-based therapeutics to lower body weight, but pathways leveraged by GIPR agonism pharmacology in the brain remain incompletely understood. We explored the role of Gipr neurons in the hypothalamus and dorsal vagal complex (DVC)—brain regions critical to the control of energy balance. Hypothalamic expression of Gipr expression was not necessary for the synergistic effect of GIPR/ agonism combined with GLP-1R co-agonism on body weight. While chemogenetic stimulation of both hypothalamic and DVC Gipr neurons suppressed food intake, activation of DVC Gipr neurons reduced ambulatory activity and induced conditioned taste avoidance, while there was no effect of a short-acting GIPR agonist (SAGIPRA). Within the DVC, Gipr neurons of the nucleus tractus solitarius (NTS), but not the area postrema (AP), projected to the parabrachial nucleus and paraventricular hypothalamic nucleusdistal brain regions. ScRNAseq and FISH analysis showed that Gipr neurons in the NTS and AP Gipr neurons were transcriptomically distinct. Peripherally-dosed fluorescent GIPR agonists (GIPRAs) revealed that GIPRA access was restricted to circumventricular organs in the CNS. Together tThese data demonstrate that while the hypothalamus, AP and NTS are key sites for Gipr expression, these subpopulations of Gipr neurons in the hypothalamus, AP and NTS differ in their connectivity, transcriptomic profile, peripheral accessibility to peripherally administered GIPRAs, and the appetite controlling pathways they employ. These results highlight the heterogeneity of the central GIPR signalling axis, and suggest that studies aimed at understandinginto the effects of GIP pharmacology on feeding behaviour should consider the interplay of multiple regulatory pathways.

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