Project description:We isolated and sequenced the complete genomes of four bacteria from the gut contents of a spiny ant (Polyrhachis lamellidens) sampled from a primeval beech forest in Yamagata Prefecture, Japan. The isolates belong to the genera Tsukamurella, Enterococcus, Lysinibacillus, and Streptomyces and provide insights into the functional roles of microbiomes of ants.