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Single-cell RNA sequencing of small intestine neurons in Hirschsprung disease mice

ABSTRACT: Single-cell RNA sequencing of small intestine neurons in Hirschsprung disease mice

PROVIDER: PRJNA821185 | ENA |

REPOSITORIES: ENA

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Single-cell RNA sequencing of small intestine neurons in Hirschsprung disease mice

Project description:This study was undertaken to identify gene expression changes in enteric neurons of Hirschsprung disease mice. Methods: We performed single-cell RNA sequencing of enteric nervous system cells isolated from the small intestine of Ednrb-knockout mice at age P14. Results: Through comparisons with published datasets of WT small intestine enteric neurons, we identify a missing neuronal population in the ganglionated small intestine of Hirschsprung disease mice. Conclusion: Single-cell RNA sequencing permits identification of cellular perturbations in Hirschsprung disease models.

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