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Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids [mRNASeq]

ABSTRACT: Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids [mRNASeq]

PROVIDER: PRJNA821474 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18534487_1.fastq.gz	Fastqsanger.gz
	SRR18534487_2.fastq.gz	Fastqsanger.gz
	SRR18534488_1.fastq.gz	Fastqsanger.gz
	SRR18534488_2.fastq.gz	Fastqsanger.gz
	SRR18534489_1.fastq.gz	Fastqsanger.gz

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Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids

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Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids [mRNASeq3prime]

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Project description:Single cell transcriptomic study (using 10x Genomics v3 kits) of gastruloids, aggregates of mESCs, at different developmental timepoints (24h, 48h and 72h post-aggregation). mESCs, corresponding to the 0h timepoint, were also sequenced. The aim of this study was to delineate the cell state transition dynamics underlying anteroposterior symmetry breaking and germ layer formation in gastruloids. Gastruloids used in this study were generated from Bra::GFP reporter mESCs (Fehling et al., 2003).

2023-12-04 | E-MTAB-12749 | biostudies-arrayexpress

Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids [mRNASeq3prime]

Project description:We report the effect of Budesonode on embryonic stem-to-mesenchymal-like transition (esMT) and 3D Gastruloids

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Symmetry breaking in gastruloid development

Project description:Symmetry breaking in gastruloid development

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Symmetry breaking in gastruloid development

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Mannose is crucial for mesoderm specification and symmetry breaking in gastruloids

Project description:Patterning and growth are fundamental features of embryonic development that must be tightly coordinated during morphogenesis. As metabolism can control cell growth while also providing mechanistic links to developmental signalling pathways, it is ideally placed to enable this coordination. To understand how metabolism impacts early mesoderm specification, we used mouse embryonic stem (ES) cell-derived gastruloids, as these enable temporal control over metabolic manipulations and can be generated in large quantities. Gastruloids show mosaic expression of two glucose transporters,Slc2a1andSlc2a3both of which co-express with the expression of both the mesodermal markerT/Braand the neural markerSox2. To understand the significance of cellular glucose uptake in development, we used the glucose metabolism inhibitor 2-deoxy-D-glucose (2-DG). 2-DG specifically blocks the expression ofT/Brawithout affecting the expression ofSox2and abolishes axial elongation in gastruloids. Surprisingly, removing glucose completely from the medium did not phenocopy 2-DG treatment despite a significant decline in glycolytic intermediates occurring under both conditions. As 2-DG can also act as a competitive inhibitor of mannose, we added mannose together with 2-DG and found that it could rescue the mesoderm specification. Together, our results show that while mannose is crucial for mesoderm specification, the glycolytic pathway is dispensable at early stages ofT/Braexpression in gastruloids.

2024-03-11 | GSE236887 | GEO

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