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PROVIDER: PRJNA821705 | ENA |
REPOSITORIES: ENA
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Project description:We developed a stem cell-derived culture system for C. hominis using human enterocytes differentiated under air-liquid interface (ALI) conditions. Human ALI (hALI) cultures supported robust growth and complete development of C. hominis in vitro including all life cycle stages. C. hominis infection induced a strong interferon response from enterocytes, likely driven by an endogenous dsRNA virus in the parasite. Prior infection with Cryptosporidium induced type III IFN secretion and consequently blunted infection with Rotavirus, suggesting such co-infections may alter vaccine efficacy.
2023-10-02 | GSE241571 | GEO
Project description:We developed a stem cell-derived culture system for C. hominis using human enterocytes differentiated under air-liquid interface (ALI) conditions. Human ALI (hALI) cultures supported robust growth and complete development of C. hominis in vitro including all life cycle stages. C. hominis infection induced a strong interferon response from enterocytes, likely driven by an endogenous dsRNA virus in the parasite. Prior infection with Cryptosporidium induced type III IFN secretion and consequently blunted infection with Rotavirus, suggesting such co-infections may alter vaccine efficacy.
2023-10-02 | GSE241570 | GEO
Project description:Vanee2010 - Genome-scale metabolic model of
Cryptosporidium hominis (iNV213)
This model is described in the article:
A genome-scale metabolic
model of Cryptosporidium hominis.
Vanee N, Roberts SB, Fong SS, Manque
P, Buck GA.
Chem. Biodivers. 2010 May; 7(5):
1026-1039
Abstract:
The apicomplexan Cryptosporidium is a protozoan parasite of
humans and other mammals. Cryptosporidium species cause acute
gastroenteritis and diarrheal disease in healthy humans and
animals, and cause life-threatening infection in
immunocompromised individuals such as people with AIDS. The
parasite has a one-host life cycle and commonly invades
intestinal epithelial cells. The current genome annotation of
C. hominis, the most serious human pathogen, predicts 3884
genes of which ca. 1581 have predicted functional annotations.
Using a combination of bioinformatics analysis, biochemical
evidence, and high-throughput data, we have constructed a
genome-scale metabolic model of C. hominis. The model is
comprised of 213 gene-associated enzymes involved in 540
reactions among the major metabolic pathways and provides a
link between the genotype and the phenotype of the organism,
making it possible to study and predict behavior based upon
genome content. This model was also used to analyze the two
life stages of the parasite by integrating the stage-specific
proteomic data for oocyst and sporozoite stages. Overall, this
model provides a computational framework to systematically
study and analyze various functional behaviors of C. hominis
with respect to its life cycle and pathogenicity.
This model is hosted on
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and identified by:
MODEL1507180071.
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2015-07-30 | MODEL1507180071 | BioModels
Project description:Cryptosporidium hominis and parvum primarily infect intestinal epithelial cells, which, in turn, play a key role in activating and communicating with the host immune system. To determinate which genes are regulated during early infection of non-transformed human epithelial cells, human ileal mucosa was removed (from surgical specimens), placed on collagen membranes, and cultured as explants. Explant cultures were infected with C. parvum, C. hominis, or control culture medium. After 24 hrs, RNA was extracted and analyzed using Affmetrix GeneChip microarrays. Among the more prominent genes with regulated expression was Osteoprotegerin (OPG), which was increased in all of the explants at 24 hrs and further up-regulated 1.58 fold by C. parvum and 2.54 fold by C. hominis infection compared with uninfected explants. Using real time PCR, we confirmed a 3.14 and 3.79 fold increase in OPG mRNA after infection with C. parvum and C. hominis respectively. Keywords: gene expression analysis via microarray
2008-03-14 | GSE7268 | GEO
Project description:Comparative Genomics of Cryptosporidium Species
| PRJNA241336 | ENA
Project description:Comparative Genomics of Cryptosporidium Species
| PRJNA239892 | ENA
Project description:Comparative Genomics of Cryptosporidium Species
| PRJNA222837 | ENA