Project description:The study comprises a hybrid transcriptome assembly of the olm (Proteus anguinus) that was created from Illumina short-reads and ONT long-reads. The transcriptome assembly is based on samples from six organs animals: brain, gut, heart, liver, lung and skin. In addition, we used the assembled transcripts and Illumina short-reads from two to three animals per organ to profile the gene expression in the olm.
Project description:Proteus mirabilis is a leading cause of catheter-associated urinary tract infections (UTIs) and urolithiasis. The transcriptional regulator MrpJ inversely modulates two critical aspects of P. mirabilis UTI progression: fimbria-mediated attachment to the urinary tract, and flagella-mediated motility. Chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) was used for the first time in a CAUTI pathogen to probe for in vivo direct targets of MrpJ. ChIP-seq revealed 81 78 direct MrpJ targets, including genes for motility, fimbriae and a type VI secretion system (T6SS), and the putative MrpJ binding sequence ACnCnnnnnnnGnGT.
