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Transcriptome (RNA-Seq) of the stable overexpression of lncRNA XLOC013218 U251 cells

ABSTRACT: Transcriptome (RNA-Seq) of the stable overexpression of lncRNA XLOC013218 U251 cells

PROVIDER: PRJNA822649 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18584339_1.fastq.gz	Fastqsanger.gz
	SRR18584339_2.fastq.gz	Fastqsanger.gz
	SRR18584340_1.fastq.gz	Fastqsanger.gz
	SRR18584340_2.fastq.gz	Fastqsanger.gz
	SRR18584341_1.fastq.gz	Fastqsanger.gz

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Transcriptome (RNA-Seq) of the stable overexpression of lncRNA XLOC013218 U251 cells

Project description:We present RNA-Seq data obtained from U251 control cells and U251 cells with stable overexpression of lncRNA XLOC13218 to identify differentially expressed genes. The discovery of long non-coding RNAs (lncRNAs) has improved the understanding of development and progression in various cancer sub-types. However, the role of lncRNAs in temozolomide (TMZ) resistance in glioblastoma (GBM) remains largely undefined. In this present study, the differential expression of lncRNAs were identified between U87 and U87TR (TMZ-resistant) cells. LncRNA XLOC013218 (XLOC) was drastically upregulated in TMZ-resistant cells and was associated with poor prognosis in glioma. Overexpression of XLOC markedly increased TMZ resistance, promoted proliferation, and inhibited apoptosis in vitro and in vivo. In addition, RNA-seq analysis and gain-of-function or loss-of-function studies revealed that PIK3R2 was the potential target of XLOC. Mechanistically, XLOC recruited Specificity Protein 1 (Sp1) transcription factor and promoted the binding of Sp1 to the promoters of PIK3R2, which elevated the expression of PIK3R2 in both mRNA and protein levels. Finally, PIK3R2-mediated activation of the PI3K/AKT signaling pathway promoted TMZ resistance and cell proliferation, but inhibited cell apoptosis. In conclusion, these data highlight the vital role of XLOC/Sp1/PIK3R2/PI3K/AKT axis in GBM TMZ resistance.

2022-04-05 | GSE200031 | GEO

Transcriptome of U251 cells overexpression complement component 7

Project description:We identified a rare coding variant (p.K420Q) in the complement component 7 (C7) gene affecting the risk of Alzheimer's disease. To investigate the cellular effects of the mutant, we performed RNA-seq in cell line overexpression wilt-type and mutant C7. U251 glioma cells with stable expression of mutant APP (K670N/M671L) (U251-APP cells), which produce Aβ42 under Dox inducing, were used as the model cell. Total RNA of U251-APP cells overexpressing wild type and mutant C7 proteins were subjected to transcriptome sequencing using Illumina Hiseq 4000 platform.

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Expression data from PARK2 overexpression in U251 cells

Project description:PARK2 (PARKIN) is an E3 ubiquitin ligase whose dysfunction has been associated with the progression of Parkinsonism and human malignancies, and its role in cancer remains to be explored. In this study, we investigated its role in glioma. We used microarrays to detail the global programme of gene expression upon PARK2 overexpression in U251 cells U251 cells with PARK2 overexpression or control (GFP) were subjected for RNA extraction and hybridization on Affymetrix microarrays.

2014-10-03 | E-GEOD-61973 | biostudies-arrayexpress

Detection of transcriptome changes caused by overexpression of NeuroD4 in U251 glioblastoma cells

Project description:We demonstrated that overexpression of NeuroD4 can reprogram GBM cells into neuron-like cells. In order to further explore the mechanism of NeuroD4 causing this phenomenon, we performed mRNA sequencing on U251 cells infected with GFP and GFP+NeuroD4.

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mRNA expression profiling in human brain glioblastoma cell line U251 after stable knockdown the expression of linc01116

Project description:The expression profiling of a total of 34181 mRNAs in 3 paired U251 cell line, which had been stable knockdown the expression of LINC01116.

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Expression data from PARK2 overexpression in U251 cells

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Project description:The objective of the study was to examine the gene expression changes in glioma cell line U87 and U251 with LAMP2A knockdown. There were 15 samples in total- U87-1, U87-2, U87-3, U87-1812-1, U87-1812-2, U87-1812-3, U251-1, U251-2, U251-3, U251-1812-1, U251-1812-2, U251-1812-3, U251-1813-1, U251-1813-2, and U251-1813-3. U87-1 to U87-3 and U251--1 to U251-3 were used as the control groups (CON). U87-1812-1 to U871812-3, U251-1812-1 to U251-1812-3, and U251-1813-1 to U251-1813-3 were used as the experimental groups (shLAMP2A-1 and shLAMP2A-2). The total RNA of each sample was extracted from the stable transfected glioma cells by using TRIzol reagent. Then the RNA samples were processed for high throughput transcriptome sequencing on Illumina HiSeq 3000 platform. There were two types of libraries: the circRNA, mRNA, and lncRNA were all constructed by removing rRNA (one library, three types of RNA were analyzed together), and the insert fragment was about 300bp; the small RNA was constructed and analyzed separately, mainly microRNA of about 22bp. Results: among 60612 cleaned mRNAs, 781 were differentially expressed in U87-1812 group compared with U87 group, 146 were differentially expressed in U251-1812 group compared with U251 group, 43 were differentially expressed in U251-1813 group compared with U251 group (padj ≤ 0.05 and expression change ≥2 fold). The differential expressed genes distributed in all chromosomes. Functional annotation with GO and KEGG enrichment revealed the top functional groups including inflammation, DNA replication, cell adhesion, TNF, IL17, and axon guidance signaling pathways.

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Project description:To investigate the effect of overexpression of STING in U251 cells, we then performed gene expression profiling analysis using data obtained from RNA-seq of two groups.

2023-12-24 | GSE228008 | GEO

PD-L1 (CD274) overexpression effect on glioblastoma cell line U251

Project description:PD-L1 (CD274) overexpression effect on glioblastoma cell line U251

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RNA sequencing facilitates quantitative analysis of U251-NC and U251-COL8A1 transcriptomes

Project description:To investigate the function of COL8A1 in GSCs progression, we established U251-COL8A1 cell lines with COL8A1 overexpressed. Then we performed gene expression profiling analysis using data obtained from RNA-seq of 2 cells (U251NC and U251-COL8A1)

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