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Transcriptome sequencing of pancreas and adipose tissues in obesity-related alcohol-induced acute pancreatitis

ABSTRACT: Transcriptome sequencing of pancreas and adipose tissues in obesity-related alcohol-induced acute pancreatitis

PROVIDER: PRJNA822805 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18590157_1.fastq.gz	Fastqsanger.gz
	SRR18590157_2.fastq.gz	Fastqsanger.gz
	SRR18590158_1.fastq.gz	Fastqsanger.gz
	SRR18590158_2.fastq.gz	Fastqsanger.gz
	SRR18590159_1.fastq.gz	Fastqsanger.gz

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Transcriptome sequencing of pancreas and adipose tissues in obesity-related alcohol-induced acute pancreatitis

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Project description:Acute pancreatitis (AP) is an acute inflammation of the pancreas, mainly caused by gallstones and alcohol, and driven by changes in communication between cells. Heparin-binding proteins (HBPs) of the plasma membrane and extracellular matrix play a central role in cell communication. Therefore, we used heparin affinity proteomics to identify the extracellular HBPs in mouse normal pancreas (NP) and in a caerulein mouse model of AP. Many new HBPs (460) were discovered more than doubling their total number to 883. A number of the new HBPs are proteins with well-characterised intracellular functions, e.g. NDUFS4, NDUFS6, but which also have a documented extracellular presence with potential ‘moonlighting’ roles. The HBPs form highly interconnected protein-protein interaction networks in both NP and AP, as well as globally. Thus, HBPs may represent the most interconnected set of extracellular proteins and so those with the greatest regulatory potential. HBPs in NP are associated with biological functions such as molecular transport, cellular movement and tissue architecture that underlie pancreatic homeostasis. However, in AP HBPs are additionally associated with processes such as acute phase response signalling, complement system and mitochondrial dysfunction. By virtue of their extracellular location and heparin binding property, HBPs are easily accessible and are potential biomarkers and drug targets in AP.

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