Project description:Current study found that oral administration markedly increased the growth capacity of DRG neurons. In this dataset, we identified genes that were differentially expressed in L4/5 DRGs after oral administration of LBP
Project description:Expression of L4 DRG after sciatic nerve cut compared to contralateral uninjured DRGs. The "SAMPLE_ID" sample characteristic is a sample identifier internal to Genentech. The ID of this project in Genentech's ExpressionPlot database is PRJ0003322 Keywords: Expression profiling by array
Project description:Oral administration of Si-based agents produces large amounts of hydrogen under alkaline conditions in the intestinal tract. Hydrogen removes reactive oxygen species and reduces oxidative stress. It is also known that oxidative stress is involved in the progression of renal fibrosis. We reported that oral administration of Si- based agent suppressed renal fibrosis using UUO model. mRNA expression in Sham group, UUO group, and UUO+Si group was evaluated by RNA-seq and functional analysis, and found that fibrosis-related Gene ontology and Pathways related to fibrosis were suppressed by the Si-based agent.
Project description:We report here a systematic approach to characterize the transcriptional responses of different cells types in the dorsal root ganglion (DRG) to peripheral nerve injury using single cell RNA sequencing (scRNAseq). We compare scRNAseq datasets of lumbar DRGs form naïve mice with corresponding datasets from mice subjected to spared nerve injury (SNI) 7 or 14 days prior to analysis. SNI surgery was performed in the left and right hindleg and development of mechanical allodynia was monitored by von Frey testing relative to control mice and the baseline level. Mice were perfused with PBS prior to extraction of L3 and L4 DRGs and DRGs were enzymatically and mechanically dissociated to single cell suspension before scRNAseq. Analysis of transcriptional changes in this nerve injury-paradigm reveals a differential response at 7 days versus 14 days post injury, suggesting dynamic gene modulation over time.
Project description:To identify genes differentially expressed during the molt, we collected RNA 30-40 minutes after feeding cessation at the start of the fourth larval stage (L4) lethargus. Additional time points for RNA collection were in the mid-L4 stage, approximately four hours prior to lethargus, and in the young adult stage, four hours after lethargus. These samples were interrogated with the Affymetrix C. elegans Genome Array. A total of 1,804 gene transcripts were up regulated, and 1,088 gene transcripts were down regulated, during the L4 lethargus period compared to the L4 and Adult stages (false discovery rate (FDR) < 0.05).
Project description:we found that chronic oral (3 months) administration of nicotine had different effects on transcriptomic profiling in prefrontal forebrain cortex of wildtype (WT) and Presenilin 1/2 double knockout mice.
Project description:Glucocorticoids are first-line agents for the treatment of many eosinophil-associated disorders. However, their mechanism of action in this group of disorders remains poorly understood, including the well-known clinical observation that glucocorticoids at therapeutic doses lead to profound, transient eosinopenia within hours of administration. To gain an unbiased, genome-wide view of the early transcriptional effects of glucocorticoids on human eosinophils in vivo, and torelate them to the kinetics of glucocorticoid-induced eosinopenia, RNA sequencing was performed on purified blood eosinophils obtained before and 30, 60, and 120 minutes after administration of a single dose of oral prednisone (1 mg/kg) to healthy subjects with hypereosinophilia (hypereosinophilia of unknown significance).