Project description:To study the responses of microbial communities to short-term nitrogen addition and warming,here we examine microbial communities in mangrove sediments subjected to a 4-months experimental simulation of eutrophication with 185 g m-2 year-1 nitrogen addition (N), 3oC warming (W) and nitrogen addition*warming interaction (NW).
Project description:Hernandia nymphaeifolia is an endangered mangrove associate with high ecological, ornamental, and medicinal values. Its special combination of glycophytic and halophytic characteristics provides an ideal system for investigating salt-responsive mechanisms that may be easily extended to genetic improvement of crops with high salt tolerance. However, little is known regarding salt-responsive mechanisms in H. nymphaeifolia. In this study, we posed gradient salt treatments on H. nymphaeifolia seedlings and investigated their physiological and transcriptional reprogramming in response to salinity stress. The results revealed that hyper-salinity stress posed more adverse impacts on leaf growth, cell integrity and photosynthetic performance of H. nymphaeifolia seedlings compared to those in growing in fresh water or low salt condition, mirroring its nature as a mangrove associate. Genes associated with osmolarity sensor and regulator, ROS scavenging and ion homeostasis were differentially expressed accordingly to alleviate the destructive effects. Furthermore, our results unraveled some pivotal kinases and proteins that regulate the synergistic expression of salt-responsive genes, which may act a key role in regulating the transcriptional remodeling upon salinity stress. These findings enrich our knowledge on the molecular mechanisms underlying the phenotypic plasticity of mangrove associates, and also provide valuable genetic resources for mangrove’s conservation and potential bioengineering application in agricultural field.
Project description:we used high-throughput Illumina Genome Analyzer IIx (GAIIx) technology to sequence the small RNA transcriptomes of the mangrove species, Avicennia marina. Based on sequence similarity or the secondary structure of precursors, we have identified 193 conserved miRNAs and 26 novel miRNAs in the small RNA transcriptome of Avicennia marina.
Project description:we used high-throughput Illumina Genome Analyzer IIx (GAIIx) technology to sequence the small RNA transcriptomes of the mangrove species, Avicennia marina. Based on sequence similarity or the secondary structure of precursors, we have identified 193 conserved miRNAs and 26 novel miRNAs in the small RNA transcriptome of Avicennia marina. 1 sample
Project description:Temperature is an important factor that affects the growth and reproduction of mangrove plants. Laguncularia racemosa (L.) C.F. Gaertn is a controversial species in China, in terms of being a pioneer species for mangrove restoration and a putative invasive species occupying the natural habitats. One of its advantages is the strong resistance to low temperature, which makes L. racemose more adaptive to extreme climate change than local mangrove species. However, little is known about the regulatory mechanisms underlying the cold-stress tolerance in L. racemose, which restricted our understanding on its biological features and invasion potential. In this study, In the current study, we investigated the physiological and molecular mechanisms involved in chilling-stress adaptation in L. racemose. Freezing temperature caused damage to cell membrane system and reduced photosynthesis efficiency in L. racemose. To combat the adverse impacts, plasma membrane biosynthesis and antioxidant processes were substantially enhanced. Furthermore, we showed that there was a difference between the responses to freeze-thaw injury in L. racemose from different locations. It may provide new clues to the different genetic background between varieties of L. racemose. These novel findings could provide biochemical and genetic basis for the cultivation and restoration of L. racemose.
Project description:Generally, salt stress causes both osmotic and ionic stress. To discern the effects of osmotic and ionic specific effects on Burma mangrove transcriptome, we conducted expression profiling in 500 mM NaCl or 1M solbitol treated leaves. This study will lead to a rapid and effective selection of gene that confers high salt tolerance in transgenic plants and to a comprehensive understanding of plant stress response. Keywords: Stress response
