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Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail

ABSTRACT: Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail

PROVIDER: PRJNA824438 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR18674400_1.fastq.gz	Fastqsanger.gz
	SRR18674400_2.fastq.gz	Fastqsanger.gz
	SRR18674401_1.fastq.gz	Fastqsanger.gz
	SRR18674401_2.fastq.gz	Fastqsanger.gz
	SRR18674402_1.fastq.gz	Fastqsanger.gz

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Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [embryonic day 9 RNA-seq]

Project description:Chemical risk assessment for avian species typically depends on information from toxicity tests performed in adult birds. Early-life stage (ELS) toxicity tests have been proposed as an attractive alternative, but incorporation of these data into existing frameworks will require knowledge about the similarities/differences between ELS and adult responses. The present study uses transcriptomics to assess hepatic gene expression in ELS and adult Japanese quail following exposure to ethinylestradiol (EE2). ELS quail were dosed with 0, 3.33, and 33.3 µg EE2/g egg via air cell injection prior to incubation. Adult quail were fed a single dose of EE2 at 0, 0.5, and 5 mg/kg body weight by gavage. Liver tissue was collected from 5-6 individuals per dose group at mid-incubation for ELS quail, and 4 days after dosing for adult birds. A total of 283 and 111 differentially expressed genes (DEGs) were detected in ELS and adult quail, respectively, 16 of which were shared across life stages. Shared DEGs included estrogenic biomarkers such as vitellogenin genes and Apovitellenin-1. For the dose groups that resulted in the highest number of DEGs (ELS [3.3 µg/g]; adult [5 mg/kg]), 21 and 35 KEGG pathways were enriched, respectively. Ten of these pathways were shared between life stages, including pathways involved with signaling molecules and interaction, and endocrine system. Taken together, our results suggest conserved mechanisms of action following estrogenic exposure across two life stages, with evidence from differentially expressed genes and enriched pathways. This study contributes to the development and evaluation of toxicogenomic and ELS approaches as alternative toxicity testing methods and supports the use of the ELS test for screening estrogenic chemicals.

2022-10-01 | GSE200395 | GEO

Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [12-week old RNA-seq]

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Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [embryonic day 9 RNA-seq]

Project description:Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [embryonic day 9 RNA-seq]

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Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [12-week old RNA-seq]

Project description:Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail [12-week old RNA-seq]

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Hepatic Transcriptomic Responses to Ethinylestradiol in Two Life Stages of Japanese Quail

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-10-01 | GSE200396 | GEO

Hepatic Transcriptomic Responses to Ethinylestradiol in Embryonic Japanese Quail and Double-crested Cormorant

Project description:Species difference in susceptibility to toxicants is a critical issue in ecotoxicology. Transcriptomics is being proposed as a strategy to improve toxicity testing for regulatory risk assessment; however, different responses among species have not been extensively explored using this approach. In the present study, we identified dysregulated genes and pathways in Japanese quail (JQ) and double-crested cormorant (DCCO) embryos following exposure to ethinylestradiol (EE2). The objectives were to evaluate unique and overlapping transcriptomic responses to estrogenic exposure in the two species, and to determine whether these responses correspond to previously reported sensitivity difference at the organismal level. Exposures were conducted according to an avian egg injection protocol that we have proposed for standardization. EE2 was dissolved in dimethyl sulfoxide and injected into the air cell of eggs prior to incubation at nominal concentrations of 0, 3.33, and 33.3 µg/g egg weight. After 9-days (JQ) or 16-days (DCCO) of incubation (representing mid-incubation stage), liver tissues were collected from 5 embryos per treatment group for RNA-sequencing. Data were processed and analyzed using EcoOmicsAnalyst (www.ecoomicsanalyst.ca) and ExpressAnalyst (www.expressanalyst.ca). Following exposure to EE2, JQ and DCCO had 238 and 1987 differentially expressed genes, respectively, having 78 of them in common. The results of this study will contribute to a better understanding of the estrogenic effects of contaminants in birds, the expansion of taxonomic coverage in chemical risk assessment from model to non-model species, and the evaluation of transcriptomics and early-life stage tests as alternative toxicity testing methods. This study was conducted as a part of a large-scale Genome Canada-funded project, EcoToxChip project (www.ecotoxchip.ca).

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Hepatic Transcriptomic Responses to Ethinylestradiol in Embryonic Japanese Quail and Double-crested Cormorant

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