Project description:We performed scRNA sequencing to find transcriptional differences in early B cell populations in the bone marrow of mice born to mothers chronically infected with Schistosoma mansoni. Cite-seq was used to help identify cluster types using surface protein markers.
Project description:To investigate heterogeneity of senescence at the single-cell level, we performed scRNA-seq analysis on bone and marrow mesenchymal cells from aged mice
Project description:We enriched endothelial cells and other bone marrow cells in both fetal and adult stage to investigate Wnt signaling interaction using targeted scRNA-seq analysis. This analysis facilitate identification of sources of Wnt ligands and detection of Wnt receptor expression in bone marrow. The comparison of fetal and adult stage reveals differences of Wnt signaling in fetal and adult BM.
Project description:RATIONALE: Radiation therapy uses high-energy x-rays to damage cancer cells. Drugs used in chemotherapy use different ways to stop cancer cells from dividing so they stop growing or die. Combining chemotherapy with bone marrow transplantation may allow the doctor to give higher doses of chemotherapy drugs and kill more tumor cells.
PURPOSE: Phase II trial to study the effectiveness of bone marrow transplantation in treating patients who have hematologic cancer.
Project description:We performed scRNA sequencing to find transcriptional differences in early B cell populations in the bone marrow of mice born to mothers chronically infected with Schistosoma mansoni compared to their mothers. Cite-seq was used to help identify cluster types using surface protein markers.
Project description:The malaria parasite Plasmodium replicates and differentiates in red blood cells of its host. The erythropoietic niches (spleen and bone marrow) are important but poorly understood reservoirs of asexual replication and sexual development. We aimed to understand how the parasite adapts to its host organ and a host cell level. For this, we performed single-cell RNA-seq (scRNA-seq) analysis on host and parasite cells derived from spleen, blood and bone marrow. Organs were harvested from two infected and one uninfected mice and parasite cells were enriched to about 50% by flow sorting (infected samples only). To identify host cells by surface expression (CITE-seq), cells were stained with barcoded antibodies targeting CD44 and CD71. Droplet-based scRNA-seq of these samples was performed using 10X genomics technology and cDNA was sequenced on Illumina.