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Adipose tissue poly RNA profiles of C57BL/6N-ΔGm15551 mice.

ABSTRACT: Adipose tissue poly RNA profiles of C57BL/6N-ΔGm15551 mice.

PROVIDER: PRJNA825726 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18729922_1.fastq.gz	Fastqsanger.gz
	SRR18729922_2.fastq.gz	Fastqsanger.gz
	SRR18729923_1.fastq.gz	Fastqsanger.gz
	SRR18729923_2.fastq.gz	Fastqsanger.gz
	SRR18729924_1.fastq.gz	Fastqsanger.gz

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Adipose tissue poly RNA profiles of C57BL/6N-ΔGm15551 mice.

Project description:Cold and nutrient activated brown adipose tissue (BAT) is capable of increasing systemic energy expenditure via uncoupled respiration and secretion of endocrine factors thereby protecting mice against diet-induced obesity and improving insulin response and glucose tolerance in men. Long non-coding RNAs (lncRNAs) have recently been identified as fine tuning regulators of cellular function. While certain lncRNAs have been functionally characterised in adipose tissue, their overall contribution in the activation of BAT remains elusive. We identified lncRNAs correlating to inter- scapular brown adipose tissue (iBAT) function in high fat diet (HFD) and cold stressed mice. We focused on Gm15551 which has an adipose tissue specific expression profile, is highly upregulated during adipogenesis and downregulated by β-adrenergic activation in mature adipocytes. Albeit we performed comprehensive transcriptional and adipocyte physiology profiling in vitro and in vivo, we could not detect an effect of gain or loss of function of Gm15551.

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Mus musculus strain:C57Bl/6N

Project description:Mus musculus strain:C57Bl/6N Raw sequence reads

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Mus musculus cultivar:C57BL/6N

Project description:Mus musculus cultivar:C57BL/6N Raw sequence reads

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Mus musculus strain:C57Bl/6N

Project description:Mus musculus strain:C57Bl/6N Raw sequence reads

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Project description:To investigate the effect of aging on mitochondrial gene expression we isolated liver mitochondria from 4 12-week and 4 65-week C57BL/6N WT mice. We then isolated RNA and prepared the barcoded library using PCR-cDNA Barcoding Kit SQK-PCB109 and ran it on MinIOn R9.4.1 Flow cells.

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Brown adipose tissue from WT and SIRT1tg mice

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