Sus scrofa
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ABSTRACT:
PROVIDER: PRJNA826369 | ENA |
REPOSITORIES: ENA
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Project description:This study evaluated the effects of dietary supplementation with a glycosylated form of active vitamin D (1,25(OH)₂D₃) combined with natural triterpenes (ursolic and oleanolic acids) in hyper-prolific sows during late gestation and lactation. Twenty-four Landrace × Large White sows were assigned to three experimental groups: a control diet (CTR) and two treatment diets supplemented with 0.64 µg/kg (ACTD1) or 0.96 µg/kg (ACTD2) of glycosylated 1,25(OH)₂D₃ combined with 140 or 210 µg/kg of triterpenes, respectively. Supplementation reduced farrowing duration and backfat thickness at weaning, while improving litter weight and piglet growth performance. Plasma analyses revealed lower levels of pro-inflammatory cytokines (TNF-α, IL-1α, IL-1β) and parathyroid hormone, alongside higher concentrations of calcitriol and calcium in treated sows. Proteomic profiling indicated modulation of lipid metabolism, coagulation, and vitamin D–related proteins, confirming systemic bioavailability of the supplemented compounds and their involvement in calcium and immune regulation. Overall, the combination of glycosylated calcitriol and triterpenes enhanced productivity, calcium homeostasis, and inflammatory balance in sows, supporting its potential as a nutritional strategy for improving reproductive efficiency and health under commercial farming conditions.
2026-03-30 | PXD070245 | Pride
Project description:Background: This study investigated the effects of dietary soy isoflavone supplementation on reproductive performance, antioxidant capacity, and colostrum-derived exosomal microRNA profiles in sows, with the aim of exploring the molecular basis of maternal–offspring integrated regulation. A total of 120 Landrace × Yorkshire sows were assigned to either a control diet or a diet supplemented with 200 mg/kg soy isoflavone from gestation day 106 to lactation day 28. Reproductive performance and serum antioxidant indices were evaluated, and colostrum-derived exosomes were isolated for small RNA sequencing and bioinformatic analysis. Results: Dietary soy isoflavone supplementation significantly increased the total number of piglets born, the number of live-born piglets, litter weight at birth, and litter weight at weaning, while shortening farrowing duration (P < 0.05). In addition, soy isoflavone significantly elevated serum total antioxidant capacity and catalase activity on lactation day 21 (P < 0.05). Transmission electron microscopy, nanoparticle tracking analysis, and flow cytometry confirmed the successful isolation of colostrum-derived exosomes. Small RNA sequencing showed that most microRNAs ranged from 18 to 26 nucleotides, with a predominant peak at 22–23 nucleotides. Principal component analysis and differential expression analysis revealed that soy isoflavone markedly reshaped the microRNA cargo of colostrum-derived exosomes. Functional enrichment analysis indicated that the predicted target genes of differentially expressed microRNAs were mainly involved in transcriptional regulation, kinase-mediated signaling, inflammatory responses, and metabolic pathways, including mitogen-activated protein kinase, Ras, Rap1, endocytosis, autophagy, and adherens junction pathways. Integrated network analyses further suggested coordinated regulation of inflammatory signaling and metabolic homeostasis. Conclusions: Dietary soy isoflavone supplementation improved sow reproductive performance and antioxidant capacity, while reshaping colostrum-derived exosomal microRNA profiles and their associated regulatory networks. These findings provide a potential molecular basis for maternal–offspring integrated regulation.
2026-04-16 | GSE327773 | GEO
Project description:This study examines whether maternal low ω6:ω3 ratio diet and offspring seaweed (SW) supplementation can improve offspring immunity and performance by elucidating the effects on piglet serum proteome. A total of 16 sows were given either a standard (CR, 13:1) or low ω6:ω3 ratio diet (LR, 4:1) during pregnancy and lactation and their male weaned piglets were supplemented with SW powder (4 g/kg, SW) or not (CT) in a 21-day post-weaning (PW) diet. Four PW piglet groups were then identified based on dam and piglet treatment, namely CRCT, CRSW, LRCT, and LRSW (n = 10 each). Piglet serum collected at weaning and d21 PW were analyzed (n = 5 each) using TMT-based quantitative proteomics and validated by appropriate assays.
2023-04-17 | PXD032327 | Pride
Project description:To investigate the dynamic changes in gene expression during mammary gland development and lactation in sows, mammary tissue samples were collected from 30 multiparous crossbred half-sibling sows (Danish Landrace × Yorkshire) housed under standardized conditions. Samples were obtained at five critical physiological stages: mid-gestation (Day 70), late gestation (Day 110), early lactation (Day 2 postpartum), peak lactation (Day 10 postpartum), and early involution (Day 2 post-weaning), with six biological replicates per stage. Parenchymal mammary tissue (0.5–1 cm³) was excised from a defined location below the third pair of teats and rapidly snap-frozen in liquid nitrogen for RNA preservation. All instruments were treated with RNase inhibitors to ensure RNA integrity. The collected samples were stored at –80°C for subsequent total RNA extraction and transcriptome sequencing, providing a valuable resource for elucidating molecular mechanisms underlying mammary gland remodeling and lactation regulation in sows.
2026-05-20 | GSE295679 | GEO
Project description:Maternal stress occuring during gestation can influence the functioning of the stress and immune responses in offspring. Maternal supplementation with immunomodulatory compounds such as omega-3 polyunsaturated fatty acids may reduce inflammation associated with maternal stress, promoting offspring health and growth Sows were fed diets supplemented with microalgae or fish oil from gestation day 75 and were challenged with LPS on gestation day 112. Previously, LPS challenge in the male piglets of these sows resulted in differences in fever and cortisol responses among piglets from different maternal treatments. Therefore, the adrenal gland was chosen for microarray analysis to assess the effects of the maternal treatment on the gene expression within the hypothalamic-pituitary-adrenal axis.
2019-08-14 | GSE135782 | GEO
Project description:Exposure to intrauterine heat stress during late gestation affects offspring performance into adulthood. However, underlying mechanistic links between thermal insult in fetal life and postnatal outcomes are not completely understood. Utilizing RNA Sequencing, this study characterized the mammary gland transcriptome of heifers that were gestated under maternal conditions of heat stress or cooling, i.e., in utero heat stressed (HT) vs. in utero cooled (CL). Mammary tissues were collected from three HT and three CL heifers during their first lactation.
2018-08-30 | GSE119216 | GEO
Project description:Transcriptional profiling of Day 30 embryos (D30E) was performed. First parity sows were submitted to an ovulation-induction protocol, intermittent suckling (IS), during lactation. IS consisted of 8 h/d separation from their litters during the last 7d of a 28d lactation. During separation, sows received boar exposure. There were 3 treatments: control (C28, n=19), where piglets were weaned at D28 of lactation and were bred after weaning and two IS treatments: sows were either bred at their first induced estrus during lactation (IS21FE, n=18), or were “skipped” and bred at their second estrus (IS21SE, n= 17) which occurred after final weaning at D28. Sows were slaughtered and embryos were collected on D30 of gestation for DNA PCR sexing. Later, D30E from the same sex with similar weight were pooled for further microarray investigation.
2015-09-16 | GSE73020 | GEO
Project description:<p>Placental dysplasia critically impairs fetal growth and development by disrupting nutrient supply. Thiamine, predominantly in its bioactive form thiamine pyrophosphate, serves as an essential coenzyme in mitochondrial energy metabolism, yet its regulatory role in placental development remains elusive.</p><p>Objectives</p><p>This study systematically unraveled the metabolic network disparities between sows with different reproductive potentials and elucidates the mechanisms by which characterized metabolites enhance placental angiogenesis and nutrient transport efficiency to promote fetal development.</p><p>Methods</p><p>We initially analyzed plasma and fecal metabolite composition and fecal microbial composition of sows with high and low reproductive potential by 16S rRNA sequencing and untargeted metabolomics. Subsequently, we evaluated the effects of thiamine, the identified characterized metabolite, on the angiogenesis and nutrient transport functions of placenta and the growth and development of offspring in a rat model, as well as verified the effects of thiamine on the cell migration of placental trophoblast cells by in vitro experiment.</p><p>Results</p><p>Sows with high reproductive potential exhibit a distinct metabolic profile with significantly elevated plasma arginine and fecal thiamine levels and the abundance of gut thiamine-synthesizing bacteria increases in parallel. Maternal thiamine supplementation effectively promotes offspring growth and enhances thiamine metabolism in the maternal-placental-fetal axis. Meanwhile, thiamine improves placental function by increasing thiamine-related metabolic enzymes and acetyl-CoA content in the placenta. Furthermore, thiamine facilitates placental angiogenesis via activation of the Notch signaling pathway and initiates the Notch-PI3K/AKT cascade reaction, which regulates placental nutrient metabolism efficiency and transporter expression.</p><p>Conclusions</p><p>Maternal supplementation with thiamine during gestation and lactation promotes placental development and enhances offspring growth through activation of the Notch signaling pathway.</p>
2025-07-29 | MTBLS12795 | MetaboLights
Project description:Exposure to intrauterine heat stress during late gestation affects offspring performance into adulthood. However, underlying mechanistic links between thermal insult in fetal life and postnatal outcomes are not completely understood. Utilizing Reduced Representation Bisulfite Sequencing, this study evaluated DNA methylation of liver and mammary gland of bull calves and heifers that were gestated under maternal conditions of heat stress or cooling, i.e., in utero heat stressed (HT) vs. in utero cooled (CL). Liver samples from bull calves (CT = 5 and HT = 4) were collected at birth while mammary gland samples from heifers (CT = 3 and HT = 3) were collected during their first lactation.
2018-09-05 | GSE119445 | GEO
Project description:Maternal supplementation with thiamine during gestation and lactation promotes placental development and enhances offspring growth through activation of the Notch signaling pathway.
2025-07-30 | MTBLS12796 | MetaboLights