Testing different rRNA removal methods in 4 species of model halophilic archaea
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ABSTRACT:
PROVIDER: PRJNA826532 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR18747300_1.fastq.gz | Fastqsanger.gz | |||
| SRR18747300_2.fastq.gz | Fastqsanger.gz | |||
| SRR18747301_1.fastq.gz | Fastqsanger.gz | |||
| SRR18747301_2.fastq.gz | Fastqsanger.gz | |||
| SRR18747302_1.fastq.gz | Fastqsanger.gz |
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Project description:We tested a number of rRNA removal methods (Illumina RiboZero Plus, NEBNext, NEB Core Depletion Set with custom probes, siTools Panarchaea, siTools RiboPool) on 4 model halophile species: Halobacterium salinarum, Haloferax volcanii, Haloferax meditteranei, Haloarcula hispanica). It was found that methods using custom probes (NEB Core Depletion set with HVO probes, siTools RiboPool with HVO probes) efficiently remove rRNA in species they are targeted to, and that Panarchaea efficiently removes rRNA in all 4 tested species.
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