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ABSTRACT: Lipid droplet-related protein Perilipin regualtes lipid metabolism in Beauveria bassiana

PROVIDER: PRJNA828424 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18829239_1.fastq.gz	Fastqsanger.gz
	SRR18829239_2.fastq.gz	Fastqsanger.gz
	SRR18829240_1.fastq.gz	Fastqsanger.gz
	SRR18829240_2.fastq.gz	Fastqsanger.gz
	SRR18829241_1.fastq.gz	Fastqsanger.gz

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RNA-Seq analysis of WT and Ron1 disruption strain in Beauveria bassiana

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Revealing the Immune Response of Sitona callosus to Beauveria bassiana Infection through Integrative Analyses of Transcriptomics and Metabolomics

Project description:The legume pest Sitona callosus poses a significant threat to alfalfa growth. Due to its unique life cycle, Beauveria bassiana has emerged as an effective fungus for controlling S. callosus . We conducted a comprehensive analysis of the transcriptome and metabolome of S. callosus infected by B. bassiana. Differential expression of antifungal genes, including heat shock genes, cytochrome P450 (CYP450) genes, cathepsin proteases, and C-type lectin were identified in S. callosus. Pathway analysis revealed associations between immune genes and metabolites involved in autophagy-animal, glucagon signaling pathway, and glycerophospholipid metabolism. These findings provide valuable insights for enhancing the control efficacy of Beauveria bassiana on Sitona callosus.

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Project description:This transcriptomic analysis aims at unveiling all possible genes orchestrated by Rad6, which is evidently required for insect pathogenicity , virulence-related cellular events and UV damage repair of Beauveria bassiana

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Project description:miRNA expression in adult flies infected with the Gram-positive bacteria Micrococcus luteus or the fungi Beauveria bassiana.

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Genome-wide effect of carbon catabolic repressor 1 (Cre1) on genomic expression of Beauveria bassiana

Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by Cre1, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of cre1 deletion and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the deletion mutant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10364 genes annotated or not annotated. Among those, 1881 genes (genomic 18.149%) were dysregulated in the absence of cre1, including 1117 down-regulated and 764 up-regulated. Conclusions: Cre1 has profound impact on the genomic expression of Beauveria bassiana.

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Project description:Purpose: This transcriptomic analysis aims at unveiling all possible genes orchestrated by pspA, which is evidently required for insect pathogenicity and virulence-related cellular events of Beauveria bassiana. Methods: Total RNAs were extracted from three 3-day-old hyphal cultures (replicates) of pspA disruption and wild-type strains grown under normal culture conditionsand and subjected to deep sequencing on Illumina NovaseqTM 6000 platform. The sequence reads that passed quality filters were mapped to the genome of Beauveria bassiana. All genes differentially expressed in the disruptant versus the wild-type strain were enriched to GO function classes and KEGG pathways. Results: The resultant transcriptome comprises 10430 genes annotated or not annotated. Among those, 1818 genes (genomic 17.54%) were dysregulated in the absence of pspA, including 806 down-regulated and 1012 up-regulated. Conclusions: pspA lacking any predictable function domain has profound impact on the genomic expression of Beauveria bassiana.

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