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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type 、 ryhB-1、 ryhB-2、 ryhB-1/ryhB-2 Mutant Strains Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type 、 ryhB-1、 ryhB-2、 ryhB-1/ryhB-2 Mutant Strains Transcriptomes

PROVIDER: PRJNA828446 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18831514_1.fastq.gz	Fastqsanger.gz
	SRR18831514_2.fastq.gz	Fastqsanger.gz
	SRR18831515_1.fastq.gz	Fastqsanger.gz
	SRR18831515_2.fastq.gz	Fastqsanger.gz
	SRR18831516_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type 、 ryhB-1、 ryhB-2、 ryhB-1/ryhB-2 Mutant Strains Transcriptomes

Project description:Purpose:To investigate the effect of small RNA RyhB-1and RyhB-2 deletion of Salmonella enteritidis on genes expression of genome under simulated intestinal conditions Methods:S. Enteritidis wild type (WT) strain 50336 and mutants △ryhB-1, △ryhB-2, and △ryhB-1/ryhB-2 were grown overnight in 50 mL of the LB medium with 160 rpm shaking at 37°C under aerobic condition, washed thrice, and re-suspended with iron-limited and nutrient-limited medium. The above prepared bacterial cells were cultured for 2 h at 37°C in the anaerobic workstation. Each sample was analysed in triplicate. Total RNA of the above stains was extracted using TRIzol reagentwith DNase digestion according to the manufacturer’s instruction. Ribosome RNA was removed using a Ribo-Zero rRNA removal kit (Illumina) that leaves the mRNA. Sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (NEB), sequenced on an Illumina Hiseq 2000 platform at Beijing Novogene Bioinformatics Technology。 Results: Analysis of the RNA-Seq data identified 496, 250, and 553 DEGs in △ryhB-1, △ryhB-2, and △ryhB-1△ryhB-2, respectively, compared to the WT strain.A total of 213 genes changed in △ryhB-1, △ryhB-2, and △ryhB-1△ryhB-2 mutants. The transcriptional levels of 176 genes changed in △ryhB-1 and △ryhB-1△ryhB-2 but not in △ryhB-2. Furthermore, 14 genes were only changed in RyhB-2, 96 DEGs were only identified in △ryhB-1, and 12 DEGs were only identified in △ryhB-2 but not in △ryhB-1△ryhB-2. Moreover, 150 DEGs were only identified in △ryhB-1△ryhB-2 but neither in △ryhB-1 nor in △ryhB-2. Conclusions: A total of 213 genes could be regulated by △ryhB-1, △ryhB-2, and △ryhB-1△ryhB-2 mutants, 176 genes are regulated only by RyhB-1, 14 genes were regulated only by RyhB-2.

2022-04-23 | GSE201112 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Solanum lycopersicum bud and flower Transcriptomes

2021-02-27 | GSE164382 | GEO

Next Generation Sequencing Facilitates Quantitative Analysis of Retinoblastoma Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Retinoblastoma Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of IBP Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of CHAA Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Chenopodium quinoa Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of U87MG Cell Transcriptomes

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Next Generation Sequencing Facilitates Quantitative Analysis of Osteoblast Mineralization Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of Osteoblast Mineralization Transcriptomes

| PRJNA623508 | ENA

Next generation sequencing facilitates quantitative analysis of oncogene induced transcriptomes

Project description:Next generation sequencing facilitates quantitative analysis of oncogene induced transcriptomes

| PRJNA769402 | ENA

Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit Transcriptomes

Project description:Next Generation Sequencing Facilitates Quantitative Analysis of watermelon fruit Transcriptomes

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