Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression of the Dnmt1/3a/3b TKO ESCs which ubiquitously express full-length DNMT1(TKO_FL) or a deletion mutant DNMT1 lacking the N-terminal 602 amino acids(TKO_602).

ABSTRACT: Gene expression of the Dnmt1/3a/3b TKO ESCs which ubiquitously express full-length DNMT1(TKO_FL) or a deletion mutant DNMT1 lacking the N-terminal 602 amino acids(TKO_602).

PROVIDER: PRJNA830834 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells [DNMT3A/3B DKO]

Project description:DNA methylation is a key epigenetic modification involved in regulating gene expression and maintaining genomic integrity. Somatic patterns of DNA methylation are largely static, apart from focal dynamics at gene regulatory elements. To further advance our understanding of the role of DNA methylation in human development and disease, we inactivated all three catalytically active DNA methyltransferases in human embryonic stem cells (ESCs) using CRISPR/Cas9 genome editing. Disruption of DNMT3A or DNMT3B individually, as well as of both enzymes in tandem, creates viable, pluripotent cell lines with distinct effects on their DNA methylation landscape as assessed by whole-genome bisulfite sequencing. Surprisingly, in contrast to mouse, deletion of DNMT1 resulted in rapid cell death in human ESCs. To overcome the immediate lethality, we generated a doxycycline (DOX) responsive tTA-DNMT1* rescue line and readily obtained homozygous DNMT1 mutant lines. However, DOX-mediated repression of the exogenous DNMT1* initiates rapid, global loss of DNA methylation, followed by extensive cell death, demonstrating that DNA methylation is essential for human ESCs cultured in standard conditions. In summary, our data provide a comprehensive characterization of DNMT mutant ESCs, including single base genome-wide maps of their targets. RRBS methylation profiling of DNMT3A/3B DKO human ES cells

2015-03-30 | E-GEOD-63280 | biostudies-arrayexpress

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells [DNMT1]

2015-03-30 | E-GEOD-63279 | biostudies-arrayexpress

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells [DNMT1]

2015-03-30 | GSE63279 | GEO

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells [DNMT3A/3B DKO]

2015-03-30 | GSE63280 | GEO

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells

Project description:Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells

| PRJNA267139 | ENA

Targeted disruption of DNMT1, 3A and 3B in human embryonic stem cells [WGBS]

2015-03-30 | GSE63278 | GEO

Gene expression of the Dnmt1/3a/3b TKO ESCs which ubiquitously express full-length DNMT1(TKO_FL) or a deletion mutant DNMT1 lacking the N-terminal 602 amino acids(TKO_602).

Project description:Gene expression analysis of TKO_FL ESCs and TKO_602 ESCs(day 0), differentiated cells from each ESCs(day4 and day7).

2022-07-28 | GSE201306 | GEO

Mouse 602

Project description:Testing 602

| PRJEB13627 | ENA

Reduced representative bisulfite sequencing (RRBS) analysis for a genome-wide comparison of DNA methylation in wild-type, USP7-KO-1, DNMT3A/3B-DKO and DNMT3A/DNMT3B/USP7-TKO HeLa cell lines.

Project description:To better characterize the effects of USP7 on DNA methylation, we performed reduced representative bisulfite sequencing (RRBS) analysis for a genome-wide comparison of DNA methylation in wild-type, USP7-KO-1, DNMT3A/3B-DKO and DNMT3A/DNMT3B/USP7-TKO HeLa cell lines. RRBS analysis showed increased DNA methylation in USK7-KO cells and loss of USP7 elevates DNA methylation on pre-existing sites and de novo methylation.

2020-09-07 | GSE136159 | GEO

RNA-seq of triple knockout (TKO) mouse embryonic stem cells: Dnmt1-/-, Dnmt3a-/-, Dnmt3b-/-.

Project description:TKO mouse embryonic stem cells were cultured in serum/LIF and 2i/LIF in order to investigate the role of methylation in the transcriptional regulation of ground state cells (Clone: TKO 121, Tsumura et.al. 2006).

2020-06-19 | GSE130686 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data