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Zfp281 inhibits the pluripotent-to-totipotent state trasition in mouse embryonic stem cells

ABSTRACT: Zfp281 inhibits the pluripotent-to-totipotent state trasition in mouse embryonic stem cells

PROVIDER: PRJNA831856 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18914379_1.fastq.gz	Fastqsanger.gz
	SRR18914379_2.fastq.gz	Fastqsanger.gz
	SRR18914380_1.fastq.gz	Fastqsanger.gz
	SRR18914380_2.fastq.gz	Fastqsanger.gz
	SRR18914381_1.fastq.gz	Fastqsanger.gz

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Zfp281 inhibits the pluripotent-to-totipotent state trasition in mouse embryonic stem cells

Project description:We perform RNA-seq in sgZfp281/sgSV40 mESC to investigate the effect of Zfp281 during 2CLC transition.

2022-04-26 | GSE201478 | GEO

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [RNA-seq]

Project description:The progression and transition between the naïve, formative, and primed pluripotent states are accompanied by a sharp activation of the de novo DNA methyltransferases and the reorganization of transcriptional and epigenetic landscapes. Here we identified Zinc Finger Protein 281 (ZFP281) as an essential factor in the formative-to-primed pluripotent state transition. Using a knockout mouse model and a knockin degron cell system, we revealed that transcription of Dnmt3a/3b depends on the activity of ZFP281 in embryonic stem cells, epiblast-like cells, and epiblast stem cells. Mechanically, chromatin-bound ZFP281 and DNA hydroxylase TET1 are decreased in the formative state but recovered in the primed state to compete with DNMT3A/3B for establishing the DNA methylation and gene expression programs of primed pluripotency. In addition, chromatin occupancy of ZFP281 and TET1 depend on the R-loop structures formed at the ZFP281 target gene promoters devoid of DNA methylation. Our study demonstrates a comprehensive role of ZFP281 in modulating DNA methylation and demethylation for the establishment and maintenance of primed pluripotency.

2023-12-14 | GSE226041 | GEO

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [ChIP-seq]

2023-12-14 | GSE226040 | GEO

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [STEM-seq]

2023-12-14 | GSE227093 | GEO

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [MapR (CUT&Run)]

2023-12-14 | GSE247461 | GEO

Zfp281 Inhibits the Pluripotent-to-Totipotent State Transition in Mouse Embryonic Stem Cells.

Project description:The cell-fate transition between pluripotent and totipotent states determines embryonic development and the first cell-lineage segregation. However, limited by the scarcity of totipotent embryos, regulators on this transition remain largely elusive. A novel model to study the transition has been recently established, named the 2-cell-like (2C-like) model. The 2C-like cells are rare totipotent-like cells in the mouse embryonic stem cell (mESC) culture. Pluripotent mESCs can spontaneously transit into and out of the 2C-like state. We previously dissected the transcriptional roadmap of the transition. In this study, we revealed that Zfp281 is a novel regulator for the pluripotent-to-totipotent transition in mESCs. Zfp281 is a transcriptional factor involved in the cell-fate transition. Our study shows that Zfp281 represses transcripts upregulated during the 2C-like transition via Tet1 and consequentially inhibits mESCs from transiting into the 2C-like state. Interestingly, we found that the inhibitory effect of Zfp281 on the 2C-like transition leads to an impaired 2C-like-transition ability in primed-state mESCs. Altogether, our study reveals a novel mediator for the pluripotent-to-totipotent state transition in mESCs and provides insights into the dynamic transcriptional control of the transition.

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ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions

Project description:ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions

| PRJNA938414 | ENA

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [STEM-seq]

Project description:ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [STEM-seq]

| PRJNA943226 | ENA

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [ChIP-seq]

Project description:ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [ChIP-seq]

| PRJNA938421 | ENA

ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [RNA-seq]

Project description:ZFP281 coordinates DNMT3 and TET1 in pluripotent state transitions [RNA-seq]