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Nilaparvata lugens

ABSTRACT: Effect of fungal infection on gut microbial community in BPH

PROVIDER: PRJNA832103 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18920319.fastq.gz	Fastqsanger.gz
	SRR18920320.fastq.gz	Fastqsanger.gz

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Nilaparvata lugens

Project description:Study on the gut microbial diversity in BPH

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Comparative expression during feeding against starvation in the rice brown planthopper, Nilaparvata lugens

Project description:Nilaparvata lugens, the brown planthopper (BPH) sucks the rice phloem sap containing high sucrose to obtain carbon source. The comparative gene expression analyses were perfomed during feeding against starvation in order to determine sugar transporter and other feeding related gene expression. Young BPH females that feed rice seedlings or feed-deprived (water-supplied) for 24 hours were prepared in triplicate. Gene expression was compared in these two groups: feeding and feed-deprived.

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Comparative expression during feeding against starvation in the rice brown planthopper, Nilaparvata lugens

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proteome analysis of Nilaparvata lugens cuticle

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proteome analysis of in Nilaparvata lugens

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Project description:DNA methylation profiling of antibiotics-treated and untreated brown planthopper (BPH) insects

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Proteomic analysis of cuticle protein in Nilaparvata lugens

Project description:The brown planthopper (BPH, Nilaparvata lugens) is the most destructive pest of rice and causes serious economic damage in Asia. Understanding the composition of Nilaparvata lugens cuticle protein will help pest control. In this study, Nilaparvata lugens cuticle was disserted, and underwent shotgun MS/MS analysis.

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Nilaparvata lugens strain:Izumo 1987, Chikugo 1989

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Comparative profiling of microRNA transcriptome in different developmental phases and analysis of microRNAs associated with reproductive development in the Brown Planthopper, Nilaparvata lugens

Project description:In the present study, small RNA libraries derived from three developmental phases (1st day after eclosion, 3rd day after eclosion and 5th day after eclosion) of short-winged morphs female adults were constructed and sequenced. A total of 15,571,298, 16,362,568 and 15,147,276 raw reads were obtained from three BPH small RNA libraries, respectively. Moreover, we identified 905 miRNAs, including 263 conserved (belonging to 37 families) and 642 novel miRNAs. In addition, the expression profiles of these miRNAs were assessed across different developmental stages Specifically, 22, 19, and 12 miRNAs were differentially expressed between3d and 1d, 5d and 1d, and 5d and 3d, respectively. A total of 43 miRNAs were differentially expressed in the three developmental phases, and 7 miRNAs were validated using quantitative RT-PCR. Target Scan and miRanda predicted14568 putative targets for 43 differentially expressed miRNAs within the 3’UTRs sequences of BPH transcriptome. Moreover, GO and KEGG pathway analysis of the predicted miRNA targets further illustrate the likely roles for these differentially expressed miRNAs in reproduction.

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