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The effect of human gingival fibroblast-conditioned medium (HGF-CM) on pulp regeneration

ABSTRACT: The effect of human gingival fibroblast-conditioned medium (HGF-CM) on pulp regeneration

PROVIDER: PRJNA832119 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18920600_1.fastq.gz	Fastqsanger.gz
	SRR18920600_2.fastq.gz	Fastqsanger.gz
	SRR18920601_1.fastq.gz	Fastqsanger.gz
	SRR18920601_2.fastq.gz	Fastqsanger.gz
	SRR18920602_1.fastq.gz	Fastqsanger.gz

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The effect of human gingival fibroblast-conditioned medium (HGF-CM) on pulp regeneration

Project description:HGF-CM increases the proliferation and migration ability of DPSC (Dental pulp stem cells) in a dosage dependent manner, and facilitates the mineralization of DPSC by upregulating odotogenic genes. Although lack of in vitro evidence, DPSC and hGF-CM could be a promising combination for pulp regeneration in the future.

2022-10-29 | GSE201524 | GEO

Expression profiling in HGF (Hereditary Gingival Fibromatosis) patients

Project description:To identify dysregulated genes in HGF (Hereditary Gingival Fibromatosis) patients, expression profiling was performed using Affymetrix Human Genome U133 plus 2.0 arrays. Total RNA was extracted from gingival tissue specimens excised from two non-syndromic HGF patients, siblings in one family, and three normal controls. Quantity and integrity of the total RNA was measured and assessed by Nanodrop spectrophotometer (Thermo Fisher Scientific) and agarose gel electrophoresis. In brief, cDNA preparation, cRNA labeling, hybridization to Affymetrix Human Genome U133 plus 2.0 arrays, and chip scanning were performed in accordance with the manufacturer’s protocol (Affymetrix). *.CEL data from the raw chip scans were imported into the Partek GS 6.5 and normalized by RMA normalization algorithms. Comparisons between the HGF and control group were performed with one-way ANOVA (p≤0.05).

2019-12-31 | E-MTAB-7801 | biostudies-arrayexpress

Expression data from Human gingival fibroblast transfected with CEMP1

Project description:Cementum protein 1 (CEMP1) is involved in cell adhesion, cell differentiation, regulates the mineralization processes during cementogenesis, and selects progenitor cells from periodontal ligament. To determine the gene expression profiling induced by CEMP1 overexpression by non-osteogenic cells such as human gingival fibroblasts we performed microarrays (Human Gene 1.0 ST, Affymetrix) to identify differentially expressed transcripts (ED) between human gingival fibroblasts (HGF) and those that overexpress CEMP1 (HGF/CEMP1).

2014-09-16 | GSE53929 | GEO

Expression data from Human gingival fibroblast transfected with CEMP1

2014-09-16 | E-GEOD-53929 | biostudies-arrayexpress

Extracellular matrix derived from dental pulp stem cells promotes osteogenic differentiation of gingival fibroblasts via Wnt and Hippo signaling pathway

Project description:This study investigated the efficacy of decellularized ECM derived from human dental pulp stem cells (dECM_DPSCs) and gingival-derived mesenchymal stem cells (dECM_GSCs) as an inductive scaffold for osteogenic differentiation of GSCs. ECM was constructed from DPSCs and GSCs that were cultured in a growth medium supplemented with L-ascorbic acid (N-ECM) or osteogenic induction medium (OM-ECM). The gene expression profile was examined using a high-throughput RNA sequencing technique.

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Gene expression profile of human dental follicle cells (hDFCs) induced by conditioned medium derived from human dental pulp cells (hDPCs-CM)

Project description:The purpose of this study was to examine the effects of hDPCs-CM on osteogenic differentiation of hDFCs and to compare gene expression in hDFCs in the presence or absence of hDPCs-CM

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Palmitate-induced gene expression in human gingival fibroblasts (HGF)

Project description:DNA microarray analysis was performed to investigate the expression of genes in HGF stimulated with palmitate Type 2 diabetes (T2D) is characterized by decreased insulin sensitivity and higher concentrations of free fatty acids (FFAs) in plasma. Among FFAs, saturated fatty acids (SFAs), such as palmitate, have been proposed to promote inflammatory responses. Although many epidemiological studies have shown a link between periodontitis and T2D, little is known about the clinical significance of SFAs in periodontitis. The goal of this study is to demonstrate a potential link between the pathogenesis of periodontitis and SFAs in plasma. HGF were treated with either with 0 (1% Bovine serum albumin) or 100 ÂµM Palmitate for 36 hours (n=2 each).

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Palmitate-induced gene expression in human gingival fibroblasts (HGF)

2015-12-31 | GSE62761 | GEO

Gene expression analyses of HUVECs treated with conditioned medium derived from bone marrow mesenchymal stromal cells (BMSC-CM)

Project description:A functional vascular system is a prerequisite for bone repair as disturbed angiogenesis often causes non-union. Paracrine factors released from human bone marrow mesenchymal stromal cells (BMSCs) have angiogenic effects on endothelial cells. However, whether these paracrine factors participate in blood flow dynamics within bone capillaries remains poorly understood. Here, we used two different microfluidic designs to investigate critical steps during angiogenesis and found pronounced effects of endothelial cell proliferation as well as chemotactic and mechanotactic migration induced by BMSC conditioned medium (CM). The application of BMSC-CM in dynamic cultures demonstrates that bioactive factors in combination with fluidic flow-induced biomechanical signals significantly enhanced endothelial cell migration. Transcriptional analyses of endothelial cells demonstrate the induction of a unique gene expression profile related to tricarboxylic acid cycle and energy metabolism by the combination of BMSC-CM factors and shear stress, which opens an interesting new avenue to explore during fracture healing. Our results stress the importance of in vivo - like microenvironments simultaneously including biochemical, biomechanical and oxygen levels when investigating key events during vessel repair.

2023-01-01 | GSE208335 | GEO

Gene expression profiling of pancreatic cancer cell conditioned myoblasts.

Project description:We verified whether conditioned media (CM) from pancreatic cancer cell lines (MIAPaCa2, CAPAN-1, PANC-1, BxPC3) alter glucose metabolism and gene expression profiles (microarray experiment with a platform of 5000 skeletal muscle cDNA) in mice myoblasts. Myoblasts were incubated with control or pancreatic cancer CM for 14 and 24 hours. Lactate significantly increased in CM compared with non-conditioned myoblasts. No variations in expression levels of the main genes involved in glycolysis were found in CM myoblasts. Propionyl coenzyme A carboxylase and isocitrate dehydrogenase 3 beta genes, which encode enzymes of the tricarboxylic acid cycle, were overexpressed, while IGFIIR and VAMP5 genes were underexpressed in CM myoblasts. PAFAH1B1 and BCL-2 genes (intracellular signal transduction) and the serine protease cathepsin G (proteolysis), were overexpressed in CM myoblasts. Tyrosine accumulation in CM myoblasts suggested that proteolysis overcomes protein synthesis. Sorcin, actin alpha, troponin T1, and filamin A were underexpressed in CM myoblasts. Our findings demonstrate that pancreatic cancer cell conditioned media enhanced lactate production and induced proteolysis, possibly by altering expression levels of a large number of genes, not only those involved in protein biosynthesis and degradation or glucose metabolism, but also those involved in the tricarboxylic acid cycle and in vesicle traffic. Keywords: Mice myoblasts, pancreatic cancer cell lines (MIAPaCa2, CAPAN-1, PANC-1, BxPC3), cDNA microarrays

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