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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [cynoDRG]

ABSTRACT: Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [cynoDRG]

PROVIDER: PRJNA832266 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18925915_1.fastq.gz	Fastqsanger.gz
	SRR18925915_2.fastq.gz	Fastqsanger.gz
	SRR18925916_1.fastq.gz	Fastqsanger.gz
	SRR18925916_2.fastq.gz	Fastqsanger.gz
	SRR18925917_1.fastq.gz	Fastqsanger.gz

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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [mouseDRG]

Project description:Sensory neurons of the dorsal root ganglion (DRG) play a crucial role in maintaining tissue homeostasis by sensing and initiating responses to stimuli. While most preclinical studies of DRGs are conducted in rodents, much less is known about the mechanisms of sensory perception in primates. We generated a transcriptome atlas for mouse, guinea pig, cynomolgus monkey, and human DRGs using a framework that implements a common laboratory workflow (i.e sequencing technologies, laboratory protocols, and sample archival methods) and multiple data-integration approaches to generate accurate cross-species mappings of sensory neuron subtypes.

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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons

Project description:Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons

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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [gpDRG]

Project description:Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [gpDRG]

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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [mouseDRG]

Project description:Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [mouseDRG]

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Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [humanDRG]

Project description:Cross-species single-nuclei transcriptome atlas of dorsal root ganglion sensory neurons [humanDRG]

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RNA-Seq of single cells from the mouse lumbar dorsal root ganglion

Project description:In order to establish a consensus catalog of dorsal rott ganglion cell types, we used comprehensive transcriptome analysis of single cells for unsupervised identification and molecular classification of sensory neurons independent of any a priori knowledge of sensory subtypes. RNA-Seq was performed on 799 dissociated single cells dissected from the mouse lumbar dorsal root ganglion distributed over a total of nine 96-well plates

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Neuronal cell lines as model dorsal root ganglion neurons: a transcriptomic comparison

Project description:Aim: Dorsal root ganglion neuron-derived immortal cell lines including ND7/23 and F-11 cells have been used extensively as in vitro model systems of native peripheral sensory neurons. However, while it is clear that some sensory neuron-specific receptors and ion channels are present in these cell lines, a systematic comparison of the molecular targets expressed by these cell lines with intact peripheral neurons is lacking. Methods: we examined the expression of RNA transcripts in the human neuroblastoma-derived cell line, SH-SY5Y, and two dorsal root ganglion hybridoma cell lines, F-11 and ND7/23, using Illumina next-generation sequencing. Results: The expression profile of these three cell lines did not resemble any specific dorsal root ganglion neuron subclass. The cell lines lacked many markers for nociceptive sensory neurons, such as the transient receptor potential V1 gene, but expressed markers for both myelinated and unmyelinated neurons. Conclusion: This paper provides insights into the receptor repertoire expressed in common dorsal root ganglion neuron-derived cell lines, and illustrates the limits and potentials of these cell lines as tools for neuropharmacological exploration.

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