Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Single cell RNAseq of engineered human mini bones for the standarized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

ABSTRACT: Single cell RNAseq of engineered human mini bones for the standarized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

PROVIDER: PRJNA833413 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Single cell RNAseq of engineered human mini bones for the standarized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

Project description:We have engineered human mesenchymal cell line-derived mini-bones as a model to study healthy hematopoiesis, leukaemia and solid tumor metastasis.

2022-10-24 | GSE201879 | GEO

Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

Project description:Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

| PRJNA820759 | ENA

Engineering human mini-bones for the standardized modeling of healthy hematopoiesis, leukemia and solid tumor metastasis

Project description:Transcriptomic profiling of MSODB both before and after 21 days of chondrogenic differentiation

2022-10-24 | GSE199577 | GEO

Spatiotemporally resolved ex vivo colorectal cancer development in engineered mini-colons (scRNA-Seq)

Project description:Here we developed topobiologically complex mini-colons able to undergo tumorigenesis ex vivo by integrating microfabrication, optogenetic, and tissue engineering approaches. With this system, tumorigenic transformation can be spatiotemporally controlled by directing oncogenic activation through blue-light exposure, and emerging colon tumors can be tracked in real-time with single-cell resolution for several weeks without breaking the culture. These induced mini-colons display rich intra- and inter-tumoral diversity and recapitulate key pathophysiological hallmarks displayed by colorectal tumors in vivo. By fine-tuning cell-intrinsic and extrinsic parameters, mini-colons can be leveraged to unveil tumorigenic determinants, including dietary patterns, microbiota-derived metabolites, and pharmacological therapies.

2024-02-24 | GSE221162 | GEO

Spatiotemporally resolved ex vivo colorectal cancer development in engineered mini-colons (RNA-Seq AKP lines)

2024-02-24 | GSE221159 | GEO

Spatiotemporally resolved ex vivo colorectal cancer development in engineered mini-colons (RNA-Seq Gpx2 KD)

2024-02-24 | GSE246568 | GEO

Genome-wide Methylation Profiles of femoral bones

Project description:Genome-wide DNA methylation profiling of femoral bones obtained from young and old individuals. The Illumina Infinium HumanMethylation EPIC BeadChip was used to obtain DNA methylation profiles across approximately 850K CpGs from human femoral bones.

2020-02-26 | GSE138307 | GEO

Patient-derived mini-colons enable long-term modeling of tumor-microenvironment complexity

Project description:The compromise to reduce, replace, and refine (3Rs) animal usage in cancer research demands the development of biologically capable ex vivo cancer models. The establishment of three-dimensional organoid-based systems has paved the way in this direction by bringing some realistic in vivo features to the in vitro context. However, faithful modeling of cancer requires a degree of tissue-level organization, multi-cellular diversity, biological durability, and experimental flexibility that far exceeds the capabilities of any existing in vitro systems. Here we implemented tissue engineering and microfabrication technologies to develop topobiologically complex patient-specific avatars of colorectal cancer able to overcome those limitations. These miniature tissues consist of long-lived gut-like-shaped healthy colon epithelia (“mini-colons”) that allow the stable integration of cancer cells and their native tumor microenvironment on a platform designed for the real-time high-resolution evaluation of cellular dynamics. This provides an unprecedented repertoire of ex vivo experimental possibilities, which we illustrate through different applications, including the discovery of a cancer associated fibroblast-triggered mechanism driving colorectal cancer invasion. As a whole, our mini-colon system pushes the boundaries of ex vivo cancer research in both basic and pre-clinical settings.

2024-05-01 | GSE226723 | GEO

Transcription profiling of protective bones of the skull (parietals) and weight-bearing bones of the limb (ulnae) in rats to obtain basal gene expression levels

Project description:Analysis of basal gene expression of the protective bones of the skull (parietals) and weight-bearing bones of the limb (ulnae) Experiment Overall Design: RNA extraction from normal bone

2008-11-22 | E-GEOD-12966 | biostudies-arrayexpress

Gene Expression Profiles of Jaw and Alveolar Bones Are Uniquely Different from Those of Trunk and Limb Bones

Project description:Certain bone graft materials are employed for alveolar bone regeneration, and autografts are commonly used. Alveolar and jaw bones are developmentally derived from neural crest cells, while most trunk and limb bones are derived from mesodermal mesenchymal cells. Consequently, the chosen bone graft material is likely to have a different developmental origin than the recipient bone. We hypothesized that there are differences in the gene expression profiles and bone healing capacities between bones with different developmental origins. Therefore, we investigated these properties in the maxilla, mandible, ilium and femur. DNA microarray data revealed close homology between the gene expression profiles of the ilium and femur. The gene expressions of Wnt-1, P75, SOX10, Nestin and Musashi-1 were significantly higher in the maxilla and mandible than in the ilium and femur. The frontal bone healed faster than the parietal bone. Parietal bone defects transplanted with maxillary and mandibular bone grafts exhibited closure. Thus, it is suggested that the maxilla and mandible have different gene expression profiles from the other bones examined, and exhibit neural crest cell properties with a marked healing ability in adults. The data further suggest that jaw bones are effective as both bone graft materials and graft beds.

2011-06-30 | GSE30167 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data