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3D genome organization in the epithelial-mesenchymal transition spectrum [scHi-C_OVCA429shGRHL]

ABSTRACT: 3D genome organization in the epithelial-mesenchymal transition spectrum [scHi-C_OVCA429shGRHL]

PROVIDER: PRJNA833468 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR18982219_1.fastq.gz	Fastqsanger.gz
	SRR18982219_2.fastq.gz	Fastqsanger.gz
	SRR18982220_1.fastq.gz	Fastqsanger.gz
	SRR18982220_2.fastq.gz	Fastqsanger.gz
	SRR18982221_1.fastq.gz	Fastqsanger.gz

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3D genome organization in the epithelial-mesenchymal transition spectrum

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3D genome organization in the epithelial-mesenchymal transition spectrum [scHi-C_PEO1HEYA8]

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3D genome organization in the epithelial-mesenchymal transition spectrum [Hi-C]

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3D genome organization in the epithelial-mesenchymal transition spectrum [scHi-C_OVCA429shGRHL]

Project description:Background The plasticity along the epithelial-mesenchymal transition (EMT) spectrum has been shown to be regulated by various epigenetic repertoires. Emerging evidence of local chromatin conformation changes suggests that regulation of EMT may occur at a higher order of three-dimensional genome level. Results We perform Hi-C analysis and combine ChIP-seq data across cancer cell lines representing different EMT states. We demonstrate that the epithelial and mesenchymal genes are regulated distinctively. We find that EMT genes are regulated within their topologically associated domains (TADs), with only a subset of mesenchymal genes being influenced by A/B compartment switches, indicating topological remodelling is required in the transcriptional regulation of these genes. At the TAD level, epithelial and mesenchymal genes are associated with different regulatory trajectories. The epithelial gene-residing TADs are enriched with H3K27me3 marks in the mesenchymal-like states. The mesenchymal gene-residing TADs , which do not show enrichment of H3K27me3 in epithelial-like states, exhibit increased interaction frequencies with regulatory elements in the mesenchymal-like states. Conclusions We propose a novel workflow coupling immunofluorescence and dielectrophoresis to unravel EMT heterogeneity at single-cell resolution. The predicted three-dimensional structures of chromosome 10, harboring Vimentin, identify cell clusters of different states. Our results pioneer a novel avenue to decipher the complexities underlying the regulation of EMT and may infer the barriers of plasticity in the 3D genome context.

2022-05-08 | GSE201918 | GEO

3D genome organization in the epithelial-mesenchymal transition spectrum [scHi-C_PEO1HEYA8]

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3D genome organization in the epithelial-mesenchymal transition spectrum [Hi-C]

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3D genome organization in the epithelial-mesenchymal transition spectrum

Project description:This SuperSeries is composed of the SubSeries listed below.

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Transcriptional organization of autism spectrum disorder

Project description:Transcriptional organization of autism spectrum disorder

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Shift of EMT gradient in 3D spheroid MSCs for activation of mesenchymal niche function

Project description:This project is described about the detailed characterization of 3D spheroid mesenchymal stem cells (MSCs). Specifically, authors showed the dynamic action towards EMT in 3D MSCs and the potential of EMT-enhanced “naïve” mesenchymal phenotype.

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Dynamic interplay between structural variation and 3D chromosome organization in pancreatic cancer

Project description:We investigate the spectrum of SVs and three-dimensional (3D) chromatin architecture in human pancreatic ductal epithelial cell carcinogenesis by using the state of art long-read single molecular real time (SMRT) and high-throughput chromosome conformation capture (Hi-C) sequencing techniques. Systematic integration of matched SMRT, in situ Hi-C, and RNA-seq datasets revealed the complicated dynamic interplay of SVs and 3D chromosome organization and their impacts on gene expression. Our studies identify and focus remodeling of chromatin folding domains associated with cross boundary SVs enabling aberrant interactions between regulatory elements. Moreover, our data also demonstrate the existence of complex genomic rearrangements associated with two key driver genes CDKN2A and SMAD4, and characterize their influence on cancer related gene expression from linear view to 3D perspective.

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