Project description:The effect of Myc activation on the proteome was investigated in U2OS cells and proteome changes were combined with Ribo-seq, RNA-seq and GRO-seq analyses.
Project description:Use Global Run-On assay (GRO)-seq, chromatin immunoprecipitation (ChIP)-seq, and DNA: RNA immunoprecipitation (DRIP)-seq to investigate whether and how hNABP2 (hSSB1) regualte transcription.
Project description:To investigate the distribution of R-loop formation, we peformed R-loop mapping analysis using S9.6 antibody-based DRIP-seq in K562 cells (wild type and SF3B1K700E mutant) at 4 days after mutant allele expression We then performed metaplot and peak-based analysis using data obtained from DRIP-seq
Project description:This SuperSeries is composed of the following subset Series: GSE27900: Gene expression analysis of mesenchymal stem cells (MSC), osteoblasts and the U2OS (osteosarcoma) cell line GSE35573: ChIP-seq analysis of H3K4Me3- and H3K27Me3-marked chromatin in mesenchymal stem cells (MSCs), osteoblasts derived from MSCs and the osteosarcoma cell line U2OS Refer to individual Series
Project description:We performed ChIP-seq targeting the glucocorticoid receptor (GR) in the U2OS-GR cell line. The cell line is derived from U2OS ATTC:HTB-96 and stably transfected with an expression construct for rat GR. The cells were treated with 100 nM dexamethasone for 4 hours, washed 2x with PBS and cultured in hormone-free medium for 24 hours before harvest.
