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Transcriptomics provides novel insights into the regulatory mechanism of lncRNA HIF1A-AS1 on VSMCs

ABSTRACT: Transcriptomics provides novel insights into the regulatory mechanism of lncRNA HIF1A-AS1 on VSMCs

PROVIDER: PRJNA834151 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19040521_1.fastq.gz	Fastqsanger.gz
	SRR19040521_2.fastq.gz	Fastqsanger.gz
	SRR19040522_1.fastq.gz	Fastqsanger.gz
	SRR19040522_2.fastq.gz	Fastqsanger.gz
	SRR19040523_1.fastq.gz	Fastqsanger.gz

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Transcriptomics provides novel insights into the regulatory mechanism of lncRNA HIF1A-AS1 on VSMCs

Project description:In current study, using a lentivirus-based expression system, we observed that overexpressing HIF1A-AS1 successfully promoted apoptosis, significantly altered cell cycle distribution and greatly facilitated migration in VSMCs, further highlighting the robust promoting effects of HIF1A-AS1 to TAA. Moreover, transcriptomics was implemented to uncover its underlying mechanism. A total of 175 differentially expressed genes (DEGs) were identified, with some of them enriched in apoptosis, migration and cell cycle related pathways. Intriguingly, some DEGs were annotated in vascular development or coagulation function pathways. In view of this, we suggested HIF1A-AS1 mediated the progression of TAA by not only regulating the function of VSMC cells, but also altering vascular development or coagulation function. Lastly, RT-qPCR confirmed the veracity and reliability of RNA-sequencing results.

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