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Equine tissue miRNA profiling

ABSTRACT: Equine tissue miRNA profiling

PROVIDER: PRJNA836389 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19141461.fastq.gz	Fastqsanger.gz
	SRR19141462.fastq.gz	Fastqsanger.gz
	SRR19141463.fastq.gz	Fastqsanger.gz
	SRR19141464.fastq.gz	Fastqsanger.gz
	SRR19141465.fastq.gz	Fastqsanger.gz

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Equine tissue miRNA profiling

Project description:Purpose: Identification of miRNA expression profiles of selected horse tissues. Methods: miRNA-seq analysis was performed on lung, heart and liver samples collected from 4 horses. The miRNA libraries were constructed from total RNA using NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs) according to the manufacturer protocol. The quantification of the obtained libraries was performed on a Qubit 2.0 spectrophotometer (Invitrogen, Life Technologies), while a quality control on a TapeStation 2200 instrument (D1000 ScreenTape; Agilent). 75 single-end cycle sequencing was performed on the NextSeq 500/550 platform (Illumina) with the use of NextSeq High Output Reagents v2 (Illumina). Results: The comparison of miRNA profiles between the investigated tissues revealed 185 differentially expressed microRNAs (p adj≤0.05) in the heart samples versus the liver samples, 172 DE miRNAs (p adj ≤0.05) in the lung samples in comparison to the liver samples, and 155 in the lung samples versus the heart samples. Conclusions: Obtained results show varied miRNA expression profiles characteristics for each investigated hore tissue.

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Project description:Short-read NGS technology (SOLIDTM, Life Technologies) was used to establish a comprehensive repertoire of miRNA expressed in either equine cartilage or subchondral bone. Undamaged cartilage and subchondral bone samples from 10-month Anglo-Arabian foals affected by osteochondrosis (OC) were analyzed and compared with samples from healthy foals. Samples were also subjected or not to an experimental mechanical loading to evaluate the role of miRNAs in the regulation of mechano-transduction pathways. Epiphyseal cartilage and subchondral bone miRNome were defined, including about 300 new miRNAs. Differentially expressed miRNAs were identified between bone and cartilage from healthy and OC foals, as well as after the experimental mechanical loading, suggesting that miRNAs play a role in equine OC physiopathology and in the cellular response to biomechanical stress in cartilage and bone.

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MicroRNA expression data from gamma-oryzanol (GO) pre-incubated differentiating equine satellite cells exposed to hydrogen peroxide

Project description:Transcriptional profiling of GO-treated (24h) differentiating equine satellite cells (3rd day of differentiation) exposed to hydrogen peroxide (1h; last hour of pre-incubation with GO) compared to control GO-untreated cells. Goal was to determine the effects of GO pre-incubation on miRNA expression in equine satellite cells exposed to hydrogen peroxide.

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MicroRNA expression data from β-hydroxy-β-methylbutyrate (HMB) pre-incubated differentiating equine satellite cells (ESC) exposed to hydrogen peroxide

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