Project description:We performed RNA Sequencing of SKMEL2 human melanoma cells expressing a NT sgRNA or sgRNA against GRAMD1B to identify differentially expressed genes in the GRAMD1B KO cells.
Project description:The purpose of this experiment is to investigate the consequences of ABHD5 deletion in the heart and to evaluate HDAC4-NT gene transfer using AAV9 in ABHD5-KO.
Project description:Next-generation sequencing of RNA samples from the mice heart of Control, ABHD5-KO and ABHD5-KO transduced with AAV9 expressing HDAC4-NT (AAV9-NT)
Project description:Loss of Traf3 caused a dramatic induction of innate immune response genes. Antigen presentation, interferon response genes and genes responsible for foreign DNA and RNA recognition were strongly upregulated by deletion of Traf3, but KO of p100 in Traf3 KO samples reversed the activation of these pathways. We did not detect any stimulation by Traf3 KO of those signaling pathways classically involved in proliferation, including PI3K/AKT, JNK, TGF-beta, WNT and Hippo
Project description:Total RNA sequencing data generated from untreated wild type (NT) and vimentin knock out (KO) mouse hippocampal neural stem cells (NSCs) cultured in vitro
Project description:The aim of our study is to identify the role of FUS in shaping the transcriptome. RNA-seq of two FUS KO clones was performed and compared to wt; for each, four replicates were sequenced. RNA molecules associated with the FUS protein were determined by means of a RNA immuno-precipitation, followed by high-throughput sequencing. Total RNA was used as a control. SH-SY5Y cells were used for both experiments. RNA-seq: 4 wt samples, 4 A4 KO samples, 4 A5 KO samples. RIP-seq: 1 input control sample, 3 anti-FUS IP replicates.