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The novel role of hnRNP UL1 in human cell nucleoli

ABSTRACT: The novel role of hnRNP UL1 in human cell nucleoli

PROVIDER: PRJNA837419 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19175734.fastq.gz	Fastqsanger.gz
	SRR19175735.fastq.gz	Fastqsanger.gz
	SRR19175736.fastq.gz	Fastqsanger.gz
	SRR19175737.fastq.gz	Fastqsanger.gz
	SRR19175738.fastq.gz	Fastqsanger.gz

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The novel role of hnRNP UL1 in human cell nucleoli

Project description:hnRNPUL1 plays an important role in the cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double-strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli. Revealing its function, we figured out that hnRNP UL1 stimulates ribosomal DNA (rDNA) gene transcription. We have performed high-throughput sequencing of libraries prepared from nucleolar and cytoplasmic-nuclear RNA fractions to identify differentially expressed ribosomal RNAs in wild-type and HNRNPUL1 knockout HEK293T cells.

2022-08-25 | GSE202821 | GEO

The novel role of hnRNP UL1 in rDNA transcription and rDNA repair in human cell nucleoli

Project description:hnRNP UL1 plays an important function in cell nuclei, where it is recruited to DNA damage sites and is involved in the repair of DNA double strand breaks. Furthermore, this protein is known as a transcriptional repressor of RNA polymerase II genes. In the present study, we have shown that hnRNP UL1 is also localized in the nucleoli. Revealing its function, we figured out that hnRNP UL1 stimulates rDNA gene transcription and may be involved in the transport of the proteins between the nucleolus and the nucleoplasm. Moreover, we observed that cells with hnRNP UL1 silencing are more sensitive to DNA damage, suggesting its role in rDNA repair pathways and nucleolar genome integrity. Indeed, we confirmed that hnRNP UL1 interacts with yH2A.X, RPA32, XRCC1, and Chk1 in cell nucleoli, suggesting its involvement in repairing of DNA damages.

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Combinatorial role of two paralogous splicing factors, hnRNP L and L-like, in multiple-exon regulation of CD45 alternative splicing

Project description:The goal of this study was to investigate the role of hnRNP L-like in alternative pre-mRNA splicing in human B-cells through an RNA-Seq approach. RNA-Seq was performed in DG75 cell line with over expression of hnRNP L-like or GFP as control.

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Project description:We sought to identify alterations in RNA expression and splicing in human grey matter from the brains of people with Multiple Sclerosis (MS) where we observe mislocalization of the RNA binding protein hnRNP A1. Thus, RNA was extracted from fresh-frozen post-mortem brain samples of three people with MS and compared to RNA extracted from fresh-frozen post-mortem brain samples of three healthy controls by RNAseq. Regions of interest in human grey matter were defined by immunostaining for hnRNP A1 to confirm grey matter regions with hnRNP A1 mislocalization in MS tissue, and grey matter regions with normal hnRNP A1 localization in control tissue, and the same region from an adjacent tissue section was dissected with a razor blade and used for RNA extraction and sequencing.

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hnRNP A1 iCLIP

Project description:hnRNP A1 iCLIP

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