Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Measuring stress response upon tissue dissociation using SLAMseq in bulk and single cells

ABSTRACT: Measuring stress response upon tissue dissociation using SLAMseq in bulk and single cells

PROVIDER: PRJNA837863 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR19178800_1.fastq.gz	Fastqsanger.gz
	SRR19178800_2.fastq.gz	Fastqsanger.gz
	SRR19178801_1.fastq.gz	Fastqsanger.gz
	SRR19178801_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 58

Similar Datasets

Labelling transcriptional response to dissociation using SLAMseq

Project description:Labelling transcriptional response to dissociation using SLAMseq

| PRJNA837586 | ENA

Measuring stress response upon tissue dissociation using SLAMseq in bulk and single cells

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-12-20 | GSE202949 | GEO

Labelling transcriptional response to dissociation using SLAMseq

Project description:Transcriptional response to dissociation is known to cause artefacts in single cell data sets. We used 4sU to label newly made transcripts during dissociation in order to to later remove them from the data set

2022-12-20 | GSE202880 | GEO

Slamseq of whole neurons with perturbed mRNA degradation machinery

Project description:We perturbed mRNA degradation machinery in Ascl1-induced neurons (iNeurons) and investigated the change in mRNA half-lives. We performed SLAMseq Metabolic RNA Labeling on Mutant iNeuron line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) and on Wild Type iNeurons (WT). The Slamseq technique provided snapshots of mRNA kinetics allowing to estimate mRNA half-lives and assess the effect of mRNA degradation machinery on the level of mRNA stability.

2023-07-12 | E-MTAB-9545 | biostudies-arrayexpress

Slamseq of whole mouse primary cortical neurons (mPCN) with perturbed mRNA degradation machinery

Project description:We perturbed mRNA degradation machinery in mouse primary cortical neurons (mPCN) and investigated the change in mRNA half-lives. We performed SLAMseq Metabolic RNA Labeling on Mutant mPCN line harbouring a ponasteroneA-inducible heterozygous dominant-negative Caf1 (dnCaf1) and on GFP-transfected mPCNs. The Slamseq technique provided snapshots of mRNA kinetics allowing to estimate mRNA half-lives.

2022-11-14 | E-MTAB-11575 | biostudies-arrayexpress

SlamSeq of subcellular compartments in mouse primary cortical neurons

Project description:We performed SlamSeq (thiol(SH)-linked alkylation for metabolic sequencing) to estimate mRNA half-lives in subcellular compartments (neurites, soma-cytoplasm and nucleus) of primary cortical neurons.

2023-07-12 | E-MTAB-12888 | biostudies-arrayexpress

Slamseq of subcellular compartments in neurons

Project description:We performed a SLAMseq Metabolic RNA Labeling on neuronal subcellular compartments, e.g. neurites and soma, derived from Ascl1-induced neurons (iNeurons). This experimental approach provides a snapshot of mRNA kinetics which allows to estimate the half-lives of mRNAs. These data was used to investigate the influence of mRNA degradation machinery in both neuronal compartments.

2023-07-12 | E-MTAB-9400 | biostudies-arrayexpress

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

| PRJNA631686 | ENA

ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

Project description:ACME dissociation: a versatile cell fixation-dissociation method for single-cell transcriptomics

| PRJNA702503 | ENA

Slamseq of subcellular compartments in neurons

Project description:Slamseq of subcellular compartments in neurons

| PRJEB39641 | ENA