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Assessment of genomic location of acetylation of lysine 27 of histone H3 in Osteoclast Precursor Cells [ChIP_seq_H3K27ac]

ABSTRACT: Assessment of genomic location of acetylation of lysine 27 of histone H3 in Osteoclast Precursor Cells [ChIP_seq_H3K27ac]

PROVIDER: PRJNA841503 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19355645.fastq.gz	Fastqsanger.gz
	SRR19355646.fastq.gz	Fastqsanger.gz
	SRR19355647.fastq.gz	Fastqsanger.gz
	SRR19355648.fastq.gz	Fastqsanger.gz
	SRR19355649.fastq.gz	Fastqsanger.gz

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Assessment of genomic location of acetylation of lysine 27 of histone H3 in Osteoclast Precursor Cells [ChIP_seq_H3K27ac]

Project description:To understand the pathogenic mechanisms of bone erosion in rheumatoid arthritis (RA), we investigated osteoclast-specific epigenetic programs, RANKL-responsive super-enhancers (SEs) and SE-associated enhancer RNAs (SE-eRNAs) in human osteoclasts. This dataset represent histone modification encoding active enhancers genome-wide.

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Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling

Project description:Supplemental data for the article: Characterization of functional reprogramming during osteoclast development using quantitative proteomics and mRNA profiling Eunkyung An, Manikandan Narayanan, and Aleksandra Nita-Lazar* *corresponding author: Cellular Networks Proteomics Unit Laboratory of Systems Biology National Institute of Allergy and Infectious Diseases National Institutes of Health Bethesda, Maryland, 20892, USA Tel. +1 301-451-4394 Fax: +1 301-480-5170 E-mail: nitalazarau@niaid.nih.gov This dataset includes: 1. Raw LC-MS(/MS) spectra (*.raw), and 2. The output from data analyses using IP2 (Intergrated Proteomics Application, San Diego, CA) searched against the UniProt_mouse_01-18-2011 set of protein sequences (normal + reversed). Note that the version of IP2 that was used could only analyze two SILAC channels at a time, so two analyses were performed (light-medium, light-heavy) (the current version can analyze 3-plex SILAC all together in one analysis). Also, IP2 was run using two criteria (1 or 2 peptides per protein). Lanes D and E were lanes of an SDS-PAGE gel, and each was 3-plex SILAC: Lane D: Light (Osteoclast Precursor), Medium (Mature Osteoclast), Heavy (Intermediate Osteoclast) Lane E: Light (Osteoclast Precursor), Medium (Intermediate Osteoclast), Heavy (Mature Osteoclast)

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