Ontology highlight
ABSTRACT:
PROVIDER: PRJNA841746 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR19369617_1.fastq.gz | Fastqsanger.gz | |||
| SRR19369617_2.fastq.gz | Fastqsanger.gz | |||
| SRR19369618_1.fastq.gz | Fastqsanger.gz | |||
| SRR19369618_2.fastq.gz | Fastqsanger.gz | |||
| SRR19369619_1.fastq.gz | Fastqsanger.gz |
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Project description:Background: The soil environment is responsible for sustaining most terrestrial plant life on earth, yet we know surprisingly little about the important functions carried out by diverse microbial communities in soil. Soil microbes that inhabit the channels of decaying root systems, the detritusphere, are likely to be essential for plant growth and health, as these channels are the preferred locations of new root growth. Understanding the microbial metagenome of the detritusphere and how it responds to agricultural management such as crop rotations and soil tillage will be vital for improving global food production. Methods: The rhizosphere soils of wheat and chickpea growing under + and - decaying root were collected for metagenomics sequencing. A gene catalogue was established by de novo assembling metagenomic sequencing. Genes abundance was compared between bulk soil and rhizosphere soils under different treatments. Conclusions: The study describes the diversity and functional capacity of a high-quality soil microbial metagenome. The results demonstrate the contribution of the microbiome from decaying root in determining the metagenome of developing root systems, which is fundamental to plant growth, since roots preferentially inhabit previous root channels. Modifications in root microbial function through soil management, can ultimately govern plant health, productivity and food security.
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Project description:True morels (Morchella spp., Morchellaceae, Ascomycota), a delicious edible mushroom, has rapidly expanded in recent years, especially in China. However, a severe disease of morels, red fruitbody disease, led to very low production of fruiting bodies. The cause reason and the mechanisms under red fruitbody are unclear. Herein, we integrated the transcriptomics and metabolomics data of M. sextelata from red fruitbody group (R) and normal group (N), which was artificial cultivation in Fujian province, China. Transcriptome data revealed the differentially expressed genes (DEGs) between R group and N group were significantly enriched in the pathways of tyrosine metabolism, riboflavin metabolism, and glycerophospholipid metabolism. Similarly, the differential accumulated metabolites (DAMs) were mainly assigned to metabolism categories, including tyrosine metabolism, biosynthesis of plant secondary metabolites, biosynthesis of amino acids, and others. Then, combined analysis of the transcriptome data and metabolome traits revealed that the most enriched pathway was tyrosine metabolism, followed by ABC transporters, alanine, aspartate and glutamate metabolism, and others. In summary, this integration of transcriptomics and metabolomics data of M. sextelata during fruitbody redness implicated several key genes, metabolites, and pathways involved in this disease. We believe that these findings will help us understand the mechanisms under fruitbody redness of M. sextelata and provide new clues for optimizing the methods for its cultivation application.
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