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Insect gut metagenome

ABSTRACT: " Effect of binary mixture of lethal dose of Emamectin benzoate (LC25) plus azadirachtin (LC25) on gut bacterial diversity in Spodoptera frugiperda (J.E. Smitha) larvae"

PROVIDER: PRJNA842526 | ENA |

REPOSITORIES: ENA

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	SRR19428201_1.fastq.gz	Fastqsanger.gz

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Spodoptera frugiperda

Project description:Effect of binary mixture of lethal dose of Chlorantraniliprole (LC25) plus azadirachtin (LC25) on gut bacterial diversity in Spodoptera frugiperda (J.E. Smitha) larvae

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MicroRNAs Mediate Insecticide Tolerance in Spodoptera frugiperda

Project description:Spodoptera frugiperda is the world’s major agricultural pests, and has the distinctive features of high fecundity, strong migratory capacity and high resistance to most insecticides. At present, the control of S. frugiperda in China relies mainly on the spraying of chemical insecticides. MicroRNAs (miRNAs) are a class of small, single-stranded, non-coding RNAs, and play crucial regulatory roles in various physiological processes, including the insecticide resistance in insects. However, little is known about the regulatory roles of miRNAs on the resistance of S. frugiperda to insecticides. In the present research, the miRNAs that were differentially expressed after cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole treatment were analyzed by RNA-Seq. A total of 504 miRNAs were systematically identified from S. frugiperda, and 24, 22, 31 and 30 miRNAs were differentially expressed after treatments of cyantraniliprole, spinetoram, emamectin benzoate and tetraniliprole. GO and KEGG enrichment analyses were used to predict the function of differentially expressed miRNAs’ target genes. Importantly, ten miRNAs were significantly differentially expressed among the treatments of three insecticides. MiR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p were significantly down-regulated among the treatments of three insecticides by RT-qPCR. Furthermore, overexpression of miR-278-5p, miR-13b-3p, miR-10485-5p and miR-10483-5p significantly increased the mortality of S. frugiperda to cyantraniliprole and emamectin benzoate. The mortality was significantly increased with spinetoram treatment after overexpression of miR-13b-3p, miR-10485-5p and miR-10483-5p. These results suggest that miRNAs, which are differentially expressed in response to insecticides, may play a key regulatory role in the insecticide resistance in S. frugiperda.

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Project description:Effect of lethal dose of Emamectin benzoate (LC25) on gut bacterial diversity in Spodoptera frugiperda (J.E. Smith) larvae

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Transcriptomic responses to emamectin benzoate in Pacific and Atlantic Canada salmon lice Lepeophtheirus salmonis with differing levels of drug resistance

Project description:This study investigates the baseline or inducible differences in between populations of Atlantic salmon lice Lepeophtheirus salmonis with differing levels of resistance to the parasiticidal drug emamectin benzoate (EMB), as well as the induced effects of EMB exposure to Pacific salmon lice. F1 generation lice were exposed in bioassays to a dilution series of emamectin benzoate.

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Effect of HdIV or MdBV injection on the Spodoptera frugiperda hemocyte and fat body transcriptomes

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Avermectin treatment for Lepeophtheirus salmonis: Impacts on host (Salmo salar) and parasite immunophysiology

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Project description:Effects of azadirachtin on hemolymph of Spodoptera frugiperda (Lepidoptera: Noctuidae)

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