Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential gene expression in murine lung cancer in vitro vs. in vivo

ABSTRACT: Differential gene expression in murine lung cancer in vitro vs. in vivo

PROVIDER: PRJNA842716 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19420513_1.fastq.gz	Fastqsanger.gz
	SRR19420513_2.fastq.gz	Fastqsanger.gz
	SRR19420514_1.fastq.gz	Fastqsanger.gz
	SRR19420514_2.fastq.gz	Fastqsanger.gz
	SRR19420515_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 36

Similar Datasets

Differential gene expression in murine lung cancer in vitro vs. in vivo

Project description:The tumor microenvironment (TME) is incredibly important to the growth of tumors. To better understand the effect that the TME has on cancer cells, we assessed genes that were differentially expressed in murine lung tumors versus cells that were cultured in an incubator. We employed our orthotopic mouse model where murine lung cancer cells were implanted into the left lung of immunocompetent, syngeneic GFP-expressing mice of the C57BL/6 strain. After 3-4 weeks, tumors were harvested and a single cell suspension was prepared from the tumors. Using fluorescent-activated cell sorting, live (DAPI-negative), GFP-negative cells were isolated from the single cell suspensions. These GFP-negative cells constitute a pure population of tumor cells, excluding cells from the TME. RNA was isolated from these tumor cells, and submitted to RNA-seq. These are our “in vivo” samples. Concurrently, the cells from the same batch of cells that were initially injected into mice were cultured in vitro, and RNA was isolated from them and submitted to RNA-seq. These samples are our “in vitro” samples. Our study specifically utilizes 2 newly developed EML4-ALK fusion-driven murine lung cancer cell lines (EA1 and EA2 cells).

2022-05-29 | GSE204918 | GEO

Gene expression of Treponema pallidum: in vitro vs. in vivo

Project description:Gene expression of Treponema pallidum: in vitro vs. in vivo

| PRJNA770553 | ENA

Multi-modal analysis of Col1a1-GFP in vitro vs in vivo state

Project description:Multi-modal analysis of Col1a1-GFP in vitro vs in vivo state

| PRJNA814077 | ENA

Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

Project description:Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU Two kinds of 7 days post fertilization bovine embryos, in-vivo 7 days blastocysts vs. In vitro 7 days blastocysts, Biological replicates: 4 in-vitro, 4 in-vivo, protocol,extract and semen shared. Dye swap.

2012-03-31 | E-GEOD-35025 | biostudies-arrayexpress

IL7R: WT vs mutant in vitro and in vivo.

Project description:The screening of putative candidate genes downstream of IL7R-INS in vitro KSL/CLP and in vivo (depicted as B-neoplasm, Notch-T-ALL, or myeloid disorder) in comparison to WT. Five-condition experiment: WT-KSL vs. INS-KSL cells; WT-CLP vs.INS-CLP; WT/ICN vs. INS/ICN; WT-CMP vs. INS-CMP. Biological replicate: each 1, One replicate per array.

2014-01-08 | E-GEOD-51211 | biostudies-arrayexpress

Transcriptome Analysis Of Bovine Expanded Blastocysts Produced In Vivo Vs In Vitro Sof Opu

Project description:Comparison of gene expression from expanded bovine blastocysts collected 7 days after fertilization and produced in vivo vs in vitro-SOF-OPU

2012-04-01 | GSE35025 | GEO

in vitro murine iBC culture

Project description:in vitro murine iBC culture

| PRJNA873118 | ENA

mRNA-seq data for DDX3 knockdown in murine hepatocytes in vivo and in vitro

Project description:A transcriptome analysis was performed to estimate a primary response of the hepatocytes to depletion of DDX3 RNA helicase both in vitro and in vivo. We used two siRNAs with different efficacy and demonstrated protein level-dependent effects on DDX3 RNA helicase depletion in the murine liver. We found that strong reduction of DDX3 protein (>85%) leads to similar changes in vitro and in vivo – we observed deregulation of cell cycle, Wnt and cadherin pathways. However, more modest downregulation of DDX3 protein (60-65%) resulted in discordant results between gene expression in vitro and in vivo – in vitro data were close to those under strong reduction, while in vivo phenotype was weak. These results demonstrate that the level of active DDX3 protein can dramatically influence on the phenotype in vivo, which should be taken into account during drug development.

2021-06-29 | GSE178618 | GEO

mRNA-seq data for DDX3 knockdown in murine hepatocytes in vivo and in vitro

Project description:mRNA-seq data for DDX3 knockdown in murine hepatocytes in vivo and in vitro

| PRJNA739730 | ENA

Effect of long non-coding RNA LL35/Falcor depletion on gene expression in murine hepatocytes in vivo and in vitro

Project description:To determine and compare a functional role of long non-coding RNA LL35/Falcor in murine hepatocytes in vitro and in vivo we performed a transcriptome analysis of AML12 hepatocytes cell line and murine liver after LL35 depletion.

2022-06-10 | GSE203431 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data