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Gene expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

ABSTRACT: Gene expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

PROVIDER: PRJNA843679 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19446054_1.fastq.gz	Fastqsanger.gz
	SRR19446054_2.fastq.gz	Fastqsanger.gz
	SRR19446055_1.fastq.gz	Fastqsanger.gz
	SRR19446055_2.fastq.gz	Fastqsanger.gz
	SRR19446056_1.fastq.gz	Fastqsanger.gz

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miRNA expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

Project description:miRNA expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

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Gene and miRNA expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

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Gene expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

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miRNA expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

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Gene and miRNA expression analysis of erythroblasts derived from SCD CD34+ HSPCs transduced with the βAS3m/miR7m compared to control- (βAS3m/miRnt or βAS3) or mock-transduced samples

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-05-24 | GSE205074 | GEO

Effect of HES6 knockdown on gene expression in vitro differentiated megakaryocytes, erythroblasts and precursor cells, cultured from CB CD34+ HSPCs.

Project description:Cord blood CD34+ hematopoietic precursors (3 donors) were control or HES6 shRNA (HES6 knockdown) transduced and cultured for four days in media containing SCF, TPO and EPO to drive differentiation towards megakaryocytes and erythroid cells. After four days four subpopulations were sorted for RNA isolation to research the effect of HES6 knockdown on gene expression. We sorted megakaryocytes (CD41+) and early (CD71+ CD235-) and late (CD71+ CD235+) erythroblasts (CD41-) and CD34- precursor cells (CD41- CD71- CD235- CD34-).

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Erythroblasts

Project description:Erythroblasts

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Human Adult Sorted Live Cell Erythroblasts transduced with Sigma non-targeting shRNA negative control (SHC002V) with puromycin selection RNAseq

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