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Comprehensive RNA-Seq Reveals Molecular Changes in Kidney Malignancy Among People Living with HIV

ABSTRACT: Comprehensive RNA-Seq Reveals Molecular Changes in Kidney Malignancy Among People Living with HIV

PROVIDER: PRJNA844081 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19476200_1.fastq.gz	Fastqsanger.gz
	SRR19476200_2.fastq.gz	Fastqsanger.gz
	SRR19476201_1.fastq.gz	Fastqsanger.gz
	SRR19476201_2.fastq.gz	Fastqsanger.gz
	SRR19476202_1.fastq.gz	Fastqsanger.gz

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Comprehensive RNA-Seq Reveals Molecular Changes in Kidney Malignancy Among People Living with HIV

Project description:To heighten the awareness of kidney malignancy in patients with HIV infection to facilitate the early diagnosis of kidney cancer, the differentially expressed mRNAs were analyzed in this malignant tumor using RNA sequencing (RNA-seq). We identified 2962 protein-coding transcripts in HIV-associated kidney cancer. KISS1R, CAIX, and NPTX2 mRNA expression levels were specifically increased in HIV-associated kidney cancer, and UMOD and TMEM213 mRNA were decreased in most cases based on real-time PCR analyses. These findings were similar to those noted for the general population with renal cell carcinoma. Immunohistochemical staining analysis also showed that a total of 16 of 18 malignant kidney cases among PLWH exhibited positive staining for KISS1R and CAIX. Pathway analysis of the differentially expressed mRNAs in HIV-associated kidney cancer revealed that several key pathways were involved, including vascular endothelial growth factor-activated receptor activity, IgG binding, and lipopolysaccharide receptor activity. Altogether, our findings reveal the identified molecular changes in kidney malignancy, which may offer a helpful explanation for cancer progression and open up new therapeutic avenues that may decrease mortality after a cancer diagnosis among PLWH.

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Project description:HIV-related comorbidities appear to be related to chronic inflammation, a condition characterizing people living with HIV (PLWH). Prior work indicates that cannabidiol (CBD) might reduce inflammation; however, the genetics underpinning this effect are not well investigated. Our main objective is to detect gene expression alterations in human peripheral blood mononuclear cells (PBMCs) from PLWH after at least one month of CBD treatment. We collected PBMCs from three HIV-positive subjects at baseline and after CBD treatment (at least 27 days) via single-cell RNA sequencing. We obtained a coherent signature, characterized by an anti-inflammatory activity, of differentially expressed genes in myeloid cells. Our study shows how CBD is associated with alterations of gene expression in myeloid cells after CBD treatment.

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