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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIRP-Seq)

ABSTRACT: Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIRP-Seq)

PROVIDER: PRJNA844327 | ENA |

REPOSITORIES: ENA

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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIRP-Seq)

Project description:Chromatin-associated long non-coding RNAs (ca-lncRNAs) play important roles in transcriptional regulation. To comprehensively investigate the chromatin interactome of an enhancer-derived ca-lncRNA, LEENE, we performed the Chromatin isolation by RNA purification (ChIRP) to pull down LEENE-associated DNAs, which were subjected to DNA-seq. Specifically, we used 10 previously validated tiling nucleotides targeting different domains based on the predicted secondary structure. LEENE was overexpressed by adenovirus to enhance the ChIRP-seq signals.

2023-02-08 | GSE205278 | GEO

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery

Project description:Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery

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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (scRNA-Seq)

Project description:To test the function of LEENE in leene-KO mice and delineate the contribution of LEENE RNA in the ischemic response, we supplemented the leene-KO mice with human LEENE RNA and performed single cell RNA-seq. The transcriptome of the EC-enriched fractions in the ischemic muscles was profiled at the single cell level.

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Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq II)

Project description:Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of LEENE in leene-KO mice and delineate the contribution of LEENE RNA in the ischemic response, we supplemented the leene-KO mice with human LEENE RNA and profiled the transcriptomic changes in the EC-enriched fractions of the ischemic muscles.

2023-02-08 | GSE205282 | GEO

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIP-Seq)

Project description:Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (ChIP-Seq)

| PRJNA844321 | ENA

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (scRNA-Seq)

Project description:Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (scRNA-Seq)

| PRJNA844324 | ENA

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq II)

Project description:Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq II)

| PRJNA844325 | ENA

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq I)

Project description:Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq I)

| PRJNA844326 | ENA

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery (RNA-Seq I)

Project description:Vascular endothelial cells play a pivotal role in whole-body homeostasis. To test the function of human long non-coding RNA LEENE in Human umbilical vein endothelial cells (HUVEC), we inhibited LEENE by using a previously validated locked nucleic acid (LNA) GapmeR under pulsatile flow which mimics the physiological state and is characterized by elevated LEENE levels. PolyA-enriched RNA-seq was performed to examine the LEENE-regulated gene expression.

2023-02-08 | GSE205279 | GEO

Long Non-Coding RNA LEENE Promotes Angiogenesis and Ischemic Recovery

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-02-08 | GSE205287 | GEO