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Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA-seq_female)

ABSTRACT: Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA-seq_female)

PROVIDER: PRJNA845804 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR21497137.fastq.gz	Fastqsanger.gz
	SRR21497138.fastq.gz	Fastqsanger.gz
	SRR21497139.fastq.gz	Fastqsanger.gz
	SRR21497140.fastq.gz	Fastqsanger.gz
	SRR21497141.fastq.gz	Fastqsanger.gz

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Gene expression profile at the single cell level of CD45+ CD11b+ cells isolated from the brain and bone marrow of mice either untreated or conditioned and transplanted with total bone marrow.

Project description:We used single-cell RNA sequencing (scRNA-seq) to analyze the diversity of bone marrow-derived CD45+CD11b+ microglia-like cells (MGLCs) engrafted in the brain of recipient mice that were conditioned using Busulfan and PLX3397 and transplanted with total bone marrow. We compared the gene expression of MGLCs to that of developmentally-derived CD45+ CD11b+ microglia/myeloid cells isolated from the brain of recipient mice (host microglia) and untreated mice (naive microglia). We also compared the gene expression of MGLCs to that of transplant-derived CD45+ CD11b+ cells engrafted in the bone marrow (abbreviated as BM-CD11b+)

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Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA_male)

Project description:Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA_male)

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Gene expression profile at the single cell level of CD45+ CD11b+ cells isolated from the brain and bone marrow of mice either untreated or conditioned and transplanted with mouse hematopoietic stem and progenitor cells (HSPCs) expanded in culture.

Project description:We used single-cell RNA sequencing (scRNA-seq) to analyze the diversity of HSPC-derived CD45+CD11b+ microglia-like cells (MGLCs) engrafted in the brain of recipient mice that were conditioned using Busulfan and PLX3397 and transplanted intravenous with Lineage negative KIT+ SCA1+ mouse HSPCs. The HSPCs were isolated from adult C57BL/6-Tg(CAG-EGFP)131Osb/LeySopJ homozygous mice. We compared the gene expression of MGLCs to that of developmentally-derived CD45+ CD11b+ microglia/myeloid cells isolated from the brain of recipient mice (host microglia) and untreated mice (naive microglia). We also compared the gene expression of MGLCs to that of transplant-derived CD45+ CD11b+ cells engrafted in the bone marrow (abbreviated as BM-CD11b+)

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Gene expression profile at single cell level of CD11b+/CD45+ myeloid cells from the brain (scRNA-seq_female)

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