Project description:Amplicon Single Molecule Footprinting (SMF) data. SMF data is obtained by treating extracted nuclei with a GpC and a CpG methyltransferases, where binding of proteins on DNA, e.g. nucleosomes and RNA Polymerase II (Pol II), leave behind unmethylated cytosines as footprints. Data in this experiment comprises SMF data obtained from DNA methyltransferase triple knockout mouse embryonic stem cells (DNMT TKO mESCs) treated with either 500 nM triptolide for 30 minutes or DMSO as a control.
Project description:The aim of this study was to investigate the plastic colonisation process, to identify the active taxa involved in biofilm formation and the mechanisms used to initiate colonisation. To achieve this, a marine plastisphere characterised by active hydrocarbonoclastic genera was used as the inoculum for a short-term microcosm experiment using virgin low-density polyethylene as the sole carbon source. Following incubation for 1 and 2 weeks (representing early and late colonisation, respectively), a taxonomic and comparative metaproteomic approach was used to explore shifts in diversity and function.