Project description:Primary objectives: The primary objective is to investigate circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Primary endpoints: circulating tumor DNA (ctDNA) via deep sequencing for mutation detection and by whole genome sequencing for copy number analyses before start (baseline) with regorafenib and at defined time points during administration of regorafenib for treatment efficacy in colorectal cancer patients in terms of overall survival (OS).
Project description:The complete mitochondrial genome of Syrphus ribesii was determined in this study. The double-stranded circular DNA molecule was 16,530 bp in length, containing 37 typical genes: 13 protein-coding genes (PCGs), 2 rRNA genes, 22 tRNA genes, and an A + T-rich region. Thirteen PCGs were 11,196 bp in size, encoding 3720 amino acids in total. All the PCGs started with ATN, except the COI used TTG as its initiation codon. Most PCGs terminated with standard codon TAA, while the COI ended with T and the ND5 ended with TA. The lrRNA and srRNA genes were 1341 bp and 793 bp in length, respectively. The A + T-rich region harbored some typical structures characteristic of the dipterans. The phylogenetic tree showed that Syrphus ribesii was closely related to Eupeodes corollae, and the Syrphidae and Pipunculidae constituted a monophyletic group within the Syrphoidea.
