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New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens

ABSTRACT: New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens

PROVIDER: PRJNA846194 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19545300.fastq.gz	Fastqsanger.gz
	SRR19545301.fastq.gz	Fastqsanger.gz
	SRR19545302.fastq.gz	Fastqsanger.gz
	SRR19545303.fastq.gz	Fastqsanger.gz
	SRR19545304.fastq.gz	Fastqsanger.gz

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New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens

Project description:Implementation and evaluation of tests (PCR, ELISA, NGS) for the presence of viruses, viroids and phytoplasma in propagation materials of apple. Research and application of new sanitation techniques using promising antiviral substances, or cryo-knife. Confirmation of health status of sanitated cultivars including use of modern diagnostic methods such as high-throughput sequencing (NGS).

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Project description:New approaches to the production of certified varieties of apple trees using NGS diagnostics of pathogens

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Project description:The purpose of this project is to examine the effects of rootstocks on the gene expression patterns in scions of apple trees. Gene expression patterns were examined in the Gala variety grafted onto seven different, commonly used rootstocks. These trees were grown in the greenhouse to limit environmental effects. Also, gene expression profiles were examined in three different varieties (Ambrosia, Melrose,and Gala) grafted onto B.9 rootstocks grown in the field. Keywords: apple, rootstock, graft, scion

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