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Transcriptome of yw fly, L(3)mbt mutant fly and Lint-O mutant fly were performed in triplicate in Drosophila melanogaster

ABSTRACT: Transcriptome of yw fly, L(3)mbt mutant fly and Lint-O mutant fly were performed in triplicate in Drosophila melanogaster

PROVIDER: PRJNA846302 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR19546800_1.fastq.gz	Fastqsanger.gz
	SRR19546800_2.fastq.gz	Fastqsanger.gz
	SRR19546801_1.fastq.gz	Fastqsanger.gz
	SRR19546801_2.fastq.gz	Fastqsanger.gz
	SRR19546802_1.fastq.gz	Fastqsanger.gz

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Transcriptome of yw fly, L(3)mbt mutant fly and Lint-O mutant fly were performed in triplicate in Drosophila melanogaster

Project description:L(3)mbt regulates the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed ChIP-seq of L(3)mbt and a newly identified interactor in OSCs to elucidate the genome-wide localization. Then we performed RNA-seq of OSCs before and after RNAi treatment of L(3)mbt and its interactors and performed differential expression analysis.

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Tissue-specific gene expression changes in l(3)mbt mutant ovaries [germline]

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Project description:L(3)mbt regulate the transcription of germ-specific genes in somatic cells but the detailed mechanism remains unclear. We performed shotgun LC/MS/MS of L(3)mbt-IP samples and identified a new interactor in OSCs.

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Transcription profiling of mouse fetal liver cells obtained from MBT-1+/+ or MBT-1-/- embryos at E14.5

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Tissue-specific gene expression changes in l(3)mbt mutant ovaries [soma]

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m6A-RIP-seq of mRNA in yw, Mettl3, Mettl14 and Hakai flies

Project description:In order to obtain a stringent m6A methylome in Drosophila adults, we performed m6A-RIP-seq in yw control (male and female), Mettl3 (male), Mettl14 (male) and Hakai (male) mutant flies. We find that the effective m6A modification, which depends on the writer complex, is mostly distributed in 5’ UTR and near start codon in Drosophila, in contrast to the mammalian system. We define a set of high-confident m6A methylation sites shared by Mettl3, Mettl14 and Hakai, indicating that Hakai is a core component of the m6A writer complex. We also find differential methylation pattern in certain loci between male and female flies.

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