Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

RNA-seq in WT and ATF3 cKO satellite cells with 3 replicates mix

ABSTRACT: RNA-seq in WT and ATF3 cKO satellite cells with 3 replicates mix

PROVIDER: PRJNA846312 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR19508956_1.fastq.gz	Fastqsanger.gz
	SRR19508956_2.fastq.gz	Fastqsanger.gz
	SRR19508957_1.fastq.gz	Fastqsanger.gz
	SRR19508957_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

RNA-seq in WT and ATF3 cKO satellite cells with 3 replicates mix

Project description:Comparative gene expression profiling analysis of RNA-seq data for satellite cells and its ATF3 cKO.

2022-06-06 | GSE205548 | GEO

RNA-seq in WT and ATF3 iKO satellite cells with 3 replicates mix

Project description:RNA-seq in WT and ATF3 iKO satellite cells with 3 replicates mix

| PRJNA844365 | ENA

RNA-seq in WT and ATF3 iKO satellite cells with 3 replicates mix

Project description:Comparative gene expression profiling analysis of RNA-seq data for satellite cells and its ATF3-iKO.

2022-06-06 | GSE205170 | GEO

H2B CUT&RUN in WT and ATF3 iKO satellite cells

Project description:H2B binding profiling analysis of ChIP-seq data for WT and ATF3-iKO satellite cells

2022-06-06 | GSE205324 | GEO

H2B CUT&RUN in WT and ATF3 iKO satellite cells

Project description:H2B CUT&RUN in WT and ATF3 iKO satellite cells

| PRJNA844383 | ENA

Gene expression profiles in skeletal muscle satellite cells lacking Dnmt1

Project description:To identify the target genes of Dnmt1 in skeletal muscle satellite cells, we generated satellite cell specific Dnmt1 KO using Pax7-CreERT2 and Dnmt1 flox mice. After Tamoxifen (cKO) or vehicle (Control) treatment for 5 consecutive days, mice were euthanized and muscle satellite cells were collected by FACS sorting and total RNA were extracted.

2021-01-08 | GSE156978 | GEO

estuary mix

Project description:estuary mix Raw sequence reads

| PRJNA957238 | ENA

ATF3 is a novel regulator of mouse neutrophil migration.

Project description:Expression of the activating transcription factor 3 (ATF3) gene is induced by Toll-like receptor (TLR) signaling. In turn, ATF3 protein inhibits the expression of various TLR-driven pro-inflammatory genes. Given its counter-regulatory role in diverse innate immune responses, we defined the effects of ATF3 on neutrophilic airway inflammation in mice. ATF3 deletion was associated with increased lipopolysaccharide (LPS)-driven airway epithelia production of CXCL1, but not CXCL2, findings concordant with a consensus ATF3-binding site identified solely in the Cxcl1 promoter. Unexpectedly, ATF3-deficient mice did not exhibit increased airway neutrophilia after LPS challenge. Bone marrow chimeras revealed a specific reduction in ATF3-/- neutrophil recruitment to wild type lungs. In vitro, ATF3-/- neutrophils exhibited a profound chemotaxis defect. Global gene expression analysis identified ablated Tiam2 expression in ATF3-/- neutrophils. TIAM2 regulates cellular motility by activating Rac1-mediated focal adhesion disassembly. Notably, ATF3-/- and ATF3-sufficient TIAM2 knockdown neutrophils, both lacking TIAM2, exhibited increased focal complex area, along with excessive CD11b-mediated F-actin polymerization. Together, our data describe a dichotomous role for ATF3-mediated regulation of neutrophilic responses: inhibition of neutrophil chemokine production, but promotion of neutrophil chemotaxis. Ly6G+ neutrophils were purified by magnetic beads from WT or ATF3 KO bone marrow and RNA was immediately isolated for global gene expression using microarrays.

2014-04-05 | E-GEOD-53973 | biostudies-arrayexpress

ATF3 links host adaptive-response to breast cancer metastasis

Project description:Cancer-host interactions play an important role in cancer development. We identified ATF3, an adaptive-response gene, in the host to facilitate metastasis. We also demonstrated that the macrophage is one of the key cell types for host-ATF3 to function. Furthermore, matrix metalloproteases 9 is a functionally important target gene of ATF3 in co-culture assays. Gene profiling with bioinformatics analyses indicated that ATF3 downstream gene-signatures derived from tumor-associated macrophages in a mouse model can distinguish human tumor stroma from M-bM-^@M-^\distantM-bM-^@M-^] stroma in and can stratify the patients into high- versus low-risk groups. Importantly, multivariate analyses indicated that high expression of ATF3 itself in mononuclear cells within breast tumors is an independent predictor for breast cancer-specific death in a cohort of patients. Approximately 21 days after orthotopic injection of PyMT breast cancer cells into syngeneic C57BL/6 mice (WT or ATF3 KO hosts) tumors were removed (tumors were ~1 cm cubic in volume), enzymatically digested to generate single cell suspensions, stained, and sorted for F4/80 and CD45 double positive cells by FACS. Isolated cells were lysed in TRIzol, processed to generate total RNA, and gene expression analyzed by microarray. Four replicate WT TAM samples and four replicate ATF3 KO TAM samples were analyzed.

2013-07-10 | E-GEOD-36904 | biostudies-arrayexpress

Satellite Cells

Project description:Satellite Cells

| PRJNA400381 | ENA