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Peronospora belbahrii

ABSTRACT: Peronospora belbahrii overview

PROVIDER: PRJNA846389 | ENA |

REPOSITORIES: ENA

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Dataset's files

Source:

			Action	DRS
		Other
	MRBU01.dat.gz	Other
	MRBU01.fasta.gz	Fasta.gz
	MRBU01.master.dat	Other
	SRR5101900.fastq.gz	Fastqsanger.gz

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1 - 5 of 13

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Peronospora belbahrii infected Sweet Basil transcriptome timecourse

Project description:Basil downy mildew (BDM) caused by Peronospora Belbahrii leads to losses in sweet basil cultivation across the world. Though resistant cultivars of basil exist, the formation of sterile offspring and the introduction of unwanted phenotypic and chemotypic traits slows breeding. Previous work by the Simon lab at Rutgers University identified pair of sweet basil cultivars; one resistant to BDM, MRI, and one susceptible, SB22. They predicted that three genes in MRI confer increased BDM resistance. RNA from infected MRI and SB22 plants was harvested during the first 3 days of infection at 4 timepoints in order to capture as many early phases of plant-pathogen interaction as possible. The goal is to develop resistance markers for use in breeding experiments.

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Peronospora belbahrii infected Sweet Basil transcriptome timecourse

Project description:Peronospora belbahrii infected Sweet Basil transcriptome timecourse

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Transcriptomic profilling of sweet basil (Ocimum basilicum) downy mildew (Peronospora belbahrii) infection

Project description:Applying a metatranscriptomic analysis pipeline (Guo et al. 2016 Frontiers in Plant Science), we are the first to analyze the host-pathogen metatranscriptome of the basil downy mildew system. RNA-sequencing technology was utilized to gain access to the full array of expressed transcripts from both O. basilicum and P. belbahrii. This RNA-seq workflow has allowed us to identity nearly 3,000 candidate P. belbahrii genes expressed in planta, as well as 1,267 and 2,798 candidate O. basilicum genes induced or suppressed respectively under P. belbahrii infection (five days post inoculation). Up-regulated candidate genes are highly enriched for biological processes such as biotic and abiotic stress responses whereas down-regulated genes are enriched for metabolism and photosynthesis, suggesting that basil plants actively respond to pathogen infection with transcriptome reprogramming.

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Transcriptomic profilling of sweet basil (Ocimum basilicum) downy mildew (Peronospora belbahrii) infection

Project description:Transcriptomic profilling of sweet basil (Ocimum basilicum) downy mildew (Peronospora belbahrii) infection

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Rapid staining method to detect and identify downy mildew (Peronospora belbahrii) in basil.

Project description:Demand for fresh-market sweet basil continues to increase, but in 2009 a new pathogen emerged, threatening commercial field/greenhouse production and leading to high crop losses. This study describes a simple and effective staining method for rapid microscopic detection of basil downy mildew (Peronospora belbahrii) from leaves of basil (Ocimum basilicum). • Fresh leaf sections infected with P. belbahrii were placed on a microscope slide, cleared with Visikol™, and stained with iodine solution followed by one drop of 70% sulfuric acid. Cell walls of the pathogen were stained with a distinct coloration, providing a high-contrast image between the pathogen and plant. • This new staining method can be used successfully to identify downy mildew in basil, which then can significantly reduce its spread if identified early, coupled with mitigation strategies. This technique can facilitate the control of the disease, without expensive and specialized equipment.

| S-EPMC4103132 | biostudies-literature

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